Montecucco, Prof. Cesare

List of Publications (Experimental papers are followed by the list of books, of Reviews/Hypothesis/Consensus papers, and chapters).

35010 citations, H index: 94 by Google Scholar (February 2021):

  1. Stazi M, D’Este G, Mattarei A, Negro S, Lista F, Rigoni M, Megighian A and Montecucco C. An agonist of the CXCR4 receptor accelerates the recovery from the peripheral neuroparalysis induced by Taipan snake envenomation. PLOS Negl. Trop. Dis. 14, e0008547 2020 commentary in Science. ENVENOMATION Recovery from paralysis by Caroline Ash in Science 02 Oct 2020: Vol. 370, Issue 6512, pp. 71-72, DOI: 10.1126/science.370.6512.71-C



  1. Bano L, Kiel M, Sales G, Doxey AC, Mansfield MJ, Wami HT, Schiavone M, Rossetto O, Pirazzini M, Dobrindt U, Montecucco C. Genome Sequence of the Fish Brain Bacterium Clostridium tarantellae. Microbiol Resour Announc 2020 9(16): e01575-19. doi: 10.1128/MRA.01575-19


  1. Rossetto O, Montecucco C. Tables of Toxicity of Botulinum and Tetanus Neurotoxins. Toxins (Basel). 2019 11(12): pii: E686. doi: 10.3390/toxins11120686.


  1. Zanetti G, Negro S, Megighian A, Mattarei A, Lista F, Fillo S, Rigoni M, Pirazzini M, Montecucco C. A CXCR4 receptor agonist strongly stimulates axonal regeneration after damage. Ann Clin Transl Neurol. 2019 doi: 10.1002/acn3.50926.


  1. Negro S, Zanetti G, Mattarei A, Valentini A, Megighian A, Tombesi G, Zugno A, Dianin V, Pirazzini M, Fillo S, Lista F, Rigoni M, Montecucco C. An Agonist of the CXCR4 Receptor Strongly Promotes Regeneration of Degenerated Motor Axon Terminals. Cells. 2019 ;8. pii: E1183. doi: 10.3390/cells8101183.
  2. Pirazzini M, Carle S, Barth H., Rossetto O, Montecucco C. Primary resistance of human patients to botulinum neurotoxins A and B. Ann. Clin. Transl. Neurology (2018) 5: 971-975.


  1. Bano L, Tonon E, Drigo I, Pirazzini M, Guolo A, Farina G, Agnoletti F, Montecucco C. Detection of Clostridium tetani Neurotoxins Inhibited In Vivo by Botulinum Antitoxin B: Potential for Misleading Mouse Test Results in Food Controls. Toxins (Basel) (2018) 10. pii: E248. doi: 10.3390/toxins10060248.
  2. Negro S, Stazi M, Marchioretto M, Tebaldi T, Rodella U, Duregotti E, Gerke V, Quattrone A, Montecucco C, Rigoni M, Viero G. Hydrogen peroxide is a neuronal alarmin that triggers specific RNAs, local translation of Annexin A2, and cytoskeletal remodeling in Schwann cells. RNA (2018) 24:915-925. doi: 10.1261/rna.064816.117.
  3. Bano L, Drigo I, Tonon E, Pascoletti S, Puiatti C, Anniballi F, Auricchio B, Lista F, Montecucco C, Agnoletti F. Identification and characterization of Clostridium botulinum group III field strains by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Anaerobe (2017) 48:126-134. doi: 10.1016/j.anaerobe.2017.08.004.
  4. Carle S, Pirazzini M, Rossetto O, Barth H, Montecucco C. High Conservation of Tetanus and Botulinum Neurotoxins Cleavage Sites on Human SNARE Proteins SuggestsThat These Pathogens Exerted Little or No Evolutionary Pressure on Humans. Toxins (Basel) (2017) 9. pii: E404. doi: 10.3390/toxins9120404.
  5. Negro S, Lessi F, Duregotti E, Aretini P, La Ferla M, Franceschi S, Menicagli M, Bergamin E, Radice E, Thelen M, Megighian A, Pirazzini M, Mazzanti CM, Rigoni M & Montecucco C. CXCL12a/SDF-1 from perisynaptic Schwann cells promotes regeneration of injured motor axon terminals. EMBO Mol. Med (2017) 9:1000-1010. doi: 10.15252/emmm.201607257.
  6. Zornetta I, Scorzeto M, Mendes Dos Reis PV, De Lima ME, Montecucco C, Megighian A, Rossetto O. Electrophysiological Characterization of the Antarease Metalloprotease from Tityus serrulatus Venom. Toxins (Basel). (2017) Feb 27;9(3). pii: E81. doi: 10.3390/toxins9030081.
  7. Rodella U, Negro S, Scorzeto M, Bergamin E, Jalink K, Montecucco C, Yuki N, Rigoni M. Schwann cells are activated by ATP released from neurons in an in vitro cellular model of Miller Fisher syndrome. Dis. Model. Mech. (2017) Jan 6. pii: dmm.027870. doi: 10.1242/dmm.027870.
  8. Azarnia Tehran D,PirazziniM, Leka O, Mattarei A, Lista F, Binz T, Rossetto  O &  Montecucco C.  HYPERLINK “http://www.ncbi.nlm.nih.gov/pubmed/27404998” Hsp90 is involved in the entry of Clostridial Neurotoxins into the Cytosol of Nerve Terminals. Cell Microbiol. (2017) 19. doi: 10.1111/cmi.12647
  9. Zornetta I, Azarnia Tehran D, Arrigoni G, Anniballi F, Bano L, Leka O, Zanotti G, Binz T & Montecucco C. The first non Clostridial botulinum-like toxin cleaves VAMP within the juxtamembrane domain. Sci Rep. (2016) 6:30257. doi: 10.1038/srep30257.
  10. Rodella U, Scorzeto M, Duregotti E, Negro S, Dickinson BC, Chang CJ, Yuki N, Rigoni M & Montecucco C. An animal model of Miller Fisher syndrome: Mitochondrial hydrogen peroxide is produced by the autoimmune attack of nerve terminals and activates Schwann cells. Neurobiol Dis. (2016) 96:95-104.
  11. Schnell L, Mittler AK, Mattarei A, Tehran DA, Montecucco C, Barth H. Semicarbazone EGA Inhibits Uptake of Diphtheria Toxin into Human Cells and Protects Cells from Intoxication. Toxins (Basel). (2016) 15;8:101 doi: 10.3390/toxins8070221.
  12. Negro S, Bergamin E, Rodella U, Duregotti E, Scorzeto M, Jalink K, Montecucco C*, Rigoni M.*. ATP Released by Injured Neurons Activates Schwann Cells. Front Cell Neurosci. (2016) 10:134. doi: 10.3389/fncel.2016.00134.
  13. Azarnia Tehran D, Zanetti G, Leka O, Lista F, Fillo S, Binz T, Shone CC, Rossetto O, Montecucco C, Paradisi C, Mattarei A, Pirazzini M. A Novel Inhibitor Prevents the Peripheral Neuroparalysis of Botulinum Neurotoxins. Sci Rep. (2015) 5:17513. doi: 10.1038/srep17513.
  14. Duregotti E, Zanetti G, Scorzeto M, Megighian A, Montecucco C, Pirazzini M, Rigoni M. Snake and Spider Toxins Induce a Rapid Recovery of Function of Botulinum Neurotoxin Paralysed Neuromuscular Junction. Toxins (Basel) (2015) 7:5322-36. doi: 10.3390/toxins7124887.240.
  15. Schnell L, Dmochewitz-Kück L, Feigl P, Montecucco C*, Barth H*. Thioredoxin reductase inhibitor auranofin prevents membrane transport of diphtheria toxin into the cytosol and protects human cells from intoxication. Toxicon (2015) 24101(15)00109-9. doi: 10.1016/j.toxicon.
  16. Zanetti G, Azarnia Tehran D, Pirazzini M, Binz T, Shone CC, Fillo S, Lista F, Rossetto O, Montecucco C. Inhibition of botulinum neurotoxins interchain disulfide bond reduction prevents the peripheral neuroparalysis of botulism. Biochem Pharmacol. (2015) 98:522-30 doi: 10.1016/j.bcp.2015.09.023.
  17. Caccin P, Scorzeto M, Damiano N, Marin O, Megighian A, Montecucco C. The synaptotagmin juxtamembrane domain is involved in neuroexocytosis. FEBS Open Bio.(2015) 5:388-96. doi: 10.1016/j.fob.2015.04.013.
  18. Duregotti E, Scorzeto M, Negro S, Zornetta I, Dickinson BC, Chang CJ, Montecucco C*, Rigoni M* . Mitochondrial alarmins released by degenerating motor axon terminals activate perisynaptic Schwann cells. Proc Natl Acad Sci U S A. (2015) HYPERLINK “http://www.pnas.org/content/early/2015/01/15/1417108112.full.pdf+html” http://www.pnas.org/content/early/2015/01/15/1417108112.full.pdf+html

Editor’s Choice: Leslie K. Ferrarelli . Summoning Schwann cells for neuromuscular recovery. Science Signalling (2015) 8, Issue 364, p. ec35 DOI: 10.1126/scisignal.aaa9059

  1. Pirazzini M, Azarnia Tehran D, Zanetti G, Megighian A, Scorzeto M, Fillo S, Shone CC, Binz T, Rossetto O, Lista F, Montecucco C. Thioredoxin and its reductase are present on synaptic vesicles, and their inhibition prevents the paralysis induced by botulinum neurotoxins. Cell Rep. (2014) 8:1870-8. doi: 10.1016/j.celrep.2014.08.017. with highlight by: Montal M. Redox regulation of botulinum neurotoxin toxicity: therapeutic implications. Trends Mol Med. (2014) Sep 18. pii: S1471-4914(14)00142-7. doi: 10.1016/j.molmed.2014.09.005.
  2. Mora-Obando D, Fernández J, Montecucco C, Gutiérrez JM, Lomonte B. Synergism between Basic Asp49 and Lys49 Phospholipase A2 Myotoxins of Viperid Snake Venom In Vitro and In Vivo. PLoS ONE (2014) 9: e109846. doi:10.1371/journal.pone.0109846
  3. Lonati D, Giampreti A, Rossetto O, Petrolini VM, Vecchio S, Buscaglia E, Mazzoleni M, Chiara F, Aloise M, Gentilli A, Montecucco C, Coccini T, Locatelli CA. Neurotoxicity of European viperids in Italy: Pavia Poison Control Centre case series 2001-2011. Clin Toxicol (Phila). (2014) 52:269-76. doi:10.3109/15.
  4. Leka O., Vallese F., Pirazzini M., Berto P., Montecucco C.*, Zanotti G.*. Diphtheria toxin conformational switching at acidic pH. FEBS J. (2014) 281:2115-22. doi: 10.1111/febs.12783
  5. Vono M, Taccone M, Caccin P, Gallotta M, Donvito G, Falzoni S, Palmieri E, Pallaoro M, Rappuoli R, Di Virgilio F, De Gregorio E*, Montecucco C*, Seubert A. The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination. Proc Natl Acad Sci U S A. (2013) 110:21095-100. doi: 10.1073/pnas.1319784110.
  6. Pirazzini M., Henke T, Rossetto O., Mahrhold S., Krez N., Rummel A., Montecucco C.*, Binz T*. Neutralisation of specific surface carboxylates speeds up translocation of botulinum neurotoxin type B enzymatic domain. FEBS Lett. (2013) 587:3831-6.
  7. Megighian A, Zordan M, Pantano S, Scorzeto M, Rigoni M, Zanini D, Rossetto O,

Montecucco C. Evidence for a radial SNARE super-complex mediating neurotransmitter release at the Drosophila neuromuscular junction. J Cell Sci. (2013) 126:3134-40. doi: 10.1242/jcs.123802.

  1. Pirazzini M, Bordin F, Rossetto O, Shone CC, Binz T, Montecucco C. The Thioredoxin Reductase-Thioredoxin System is involved in the Entry of Tetanus and Botulinum Neurotoxins in the Cytosol of Nerve Terminals. FEBS Lett. (2013) 587:150-5. doi: 10.1016/j.febslet.2012.11.007.
  2. Colasante C, Morbiato L., Rossetto O, Pirazzini M, Molgo J. and Montecucco C. Botulinum Neurotoxin type A is Internalized and Translocates from Small Synaptic Vesicles at the Neuromuscular Junction. Mol. Neurobiol. (2013), 48:120-127. doi:10.1007/s12035-013-8423-9
  3. Silva, DS Pereira, LMG Moreira, AM Silva, F Brito,R Faria T, Zornetta I, Montecucco C, Oliveira P, Azevedo JE, Pereira PJB, Macedo-Ribeiro , S do Vale A, dos Santos NMS. The apoptogenic toxin AIP56 is a metalloprotease A-B toxin that cleaves NF-κB p65. PLOS Pathogens (2013) 9:e1003128. doi: 10.1371/journal.ppat.1003128.
  4. Pirazzini M, Rossetto O, Bertasio C, Bordin F, Shone CC, Binz T, Montecucco C. Time course and temperature dependence of the membrane translocation of tetanus and botulinum neurotoxins C and D in neurons. Biochem Biophys Res Commun. (2013) 430:38-42. doi: 10.1016/j.bbrc.2012.11.048.
  5. Eleopra R, Montecucco C, Devigili G, Lettieri C, Rinaldo S, Verriello L, Pirazzini M, Caccin P, Rossetto O. Botulinum neurotoxin serotype D is poorly effective in humans: An in vivo electrophysiological study. Clin Neurophysiol. (2013) 124:999-1004. doi: 10.1016/j.clinph.2012.11.0042


  1. Caccin P, Pellegatti P, Fernandez J, Vono M, Cintra-Francischinelli M, Lomonte B, Gutierrez JM, Di Virgilio F, Montecucco C. Why myotoxin-containing snake venoms possess powerful nucleotidases? Biochem Biophys Res Commun. (2013) :1289-93. doi: 10.1016/j.bbrc.2012.11.129.
  2. Duregotti E, Tedesco E, Montecucco C, Rigoni M. Calpains participate in nerve terminal degeneration induced by spider and snake presynaptic neurotoxins. Toxicon (2013) 64:20-8. doi: 10.1016/j.toxicon.2012.12.0072.
  3. Tonello F., Simonato M., Aita A., Pizzo P., Fernández J., Lomonte B., Gutiérrez JM and Montecucco C.. A Lys49-PLA2 Myotoxin of Bothrops asper Triggers a Rapid Death of Macrophages that Involves Autocrine Purinergic Receptor Signaling. (2012) Cell Death & Dis., (2012) 5;3:e343. doi: 10.1038/cddis.2012.68.
  4. Zornetta I. Caccin P., Fernandez J., Lomonte, B., Gutierrez JM, Montecucco C. Bothrops and Crotalus snakes induce the release of mitochondrial alarmins. PLoS Negl. Trop. Dis. (2012) 6(2):e1526, http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001526
  5. Pirazzini M, Rossetto O, Bolognese P, Shone CC, Montecucco C. Double anchorage to the membrane and intact inter-chain disulfide bond are required for the low pH induced entry of tetanus and botulinum neurotoxins into neurons. Cell. Microbiol. (2011) 13:1731- 43
  6. Megighian A, Scorzeto M, Zanini D, Pantano S, Rigoni M, Benna C, Rossetto O, Montecucco C, Zordan M. Arg206 of SNAP-25 is essential for neuroexocytosis at the Drosophila melanogaster neuromuscular junction. J Cell Sci. (2010) 123:3276-83.
  7. Zornetta I, Brandi L, Janowiak B, Dal Molin F, Tonello F, Collier RJ,Montecucco C. Imaging the cell entry of the anthrax oedema and lethal toxins with fluorescent protein chimeras. Cell. Microbiol. (2010) 12:1435- 1445.
  8. Silva-Cardoso L, Caccin P, Magnabosco A, Patron M, Targino M, Fuly A, Oliveira GA, Pereira MH, do Carmo MG, Souza AS, Silva-Neto MA, Montecucco C, Atella GC. Paralytic activity of lysophosphatidylcholine from saliva of the waterbug Belostoma anurum. J. Exp Biol. (2010) 213:3305-10.
  9. Caccin P, Magnabosco A, Tedesco E, Silva-Cardoso L, Atella GC, Montecucco C. Lysophospholipids are evolutionary ancient venom components. Toxicon. (2010) 56, 1525-1527
  10. Cintra-Francischinelli M, Caccin P, Chiavegato A, Pizzo P, Carmignoto G, Angulo Y, Lomonte B, Gutierrez JM, Montecucco C. Bothrops snake myotoxins induce a large efflux of ATP and potassium with spreading of cell damage and pain. Proc Natl Acad Sci U S A. (2010) 107:14140-14145.
  11. Cintra-Francischinelli M, Pizzo P, Angulo Y, Gutierrez JM, Montecucco C, Lomonte B. The C-terminal region of a Lys49 myotoxin mediates Ca2+ influx in C2C12 myotubes. Toxicon. (2010) 55:590-6.
  12. FOGAR P, NAVAGLIA F, BASSO D, ZAMBON CF, MOSERLE L, INDRACCOLO S, STRANGES A, GRECO E, FADI E, PADOAN A, PANTANO G, SANZARI MC, PEDRAZZOLI S, MONTECUCCO C and PLEBANI M. Heat-induced transcription of diphtheria toxin A or its variants, CRM176 and CRM197: implications for pancreatic cancer gene therapy. Cancer Gene

Ther. (2009) 17:58-68.

  1. PAOLI M., RIGONI M., KOSTER G., ROSSETTO O., MONTECUCCO C. and POSTLE A.D. Mass Spectrometry Analysis of the Phospholipase A2 Activity of Snake Presynaptic Neurotoxins in Cultured Neurons. J Neurochem. (2009) 111:737-44 .
  2. FOGOLARI F., TOSATTO S, MURARO L. and MONTECUCCO C. Electric dipole reorientation in the interaction of botulinum neurotoxins with neuronal membranes. FEBS Lett (2009) 583:2321-2325.
  3. CINTRA-FRANCISCHINELLI M., RODRIGUES-SIMIONI L, PONCE-SOTO L.A., ROSSETTO O., LOMONTE B., GUTIERREZ J.M., POZZAN T. and MONTECUCCO C. Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors. Cell Mol Life Sci. (2009) 66:1718-1728.
  4. TEDESCO E., RIGONI M., CACCIN P., GRISHIN E., ROSSETTO O. and MONTECUCCO C.. Calcium overload in nerve terminals of cultured neurons intoxicated by alpha-latrotoxin and snake PLA2 neurotoxins. Toxicon (2009) 54:138-144.
  5. ROSSI-PACCANI S., BENAGIANO M., CAPITANI N., ZORNETTA I., LADANT D., MONTECUCCO C., D’ELIOS M.M. & BALDARI C.T. The Adenylate Cyclase Toxins of Bacillus anthracis and Bordetella pertussis Promote Th2 Cell Development by Shaping T Cell Antigen Receptor Signaling. PLoS Pathogens (2009) HYPERLINK “http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000325” \t “_blank” http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000325
  6. MURARO L, TOSATTO S, MOTTERLINI L, ROSSETTO O. & MONTECUCCO C. The N-terminal half of the receptor domain of botulinum neurotoxin A binds to microdomains of the plasma membrane. Biochem Biophys Res Commun. (2009) 380:76-80
  7. CARLI L., MONTECUCCO C. & ROSSETTO O. Assay of diffusion of different botulinum neurotoxin type A formulations injected in the mouse leg. Muscle & Nerve (2009) 40: 374-380
  8. CACCIN P., ROSSETTO O. & MONTECUCCO. Neurotoxicity of inverted-cone shake lipids. Neurotoxicology (2009) 30:174-181.
  9. PUHAR A, DAL MOLIN F, HORVATH S, LADANT D, MONTECUCCO C. Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases. PLoS ONE. (2008) 3:e3564. Epub 2008 Oct 29


  1. DAL MOLIN F., FASANELLA A., SIMONATO M., GAROFOLO G., MONTECUCCO C & TONELLO F. Ratio of lethal and edema factors in rabbit systemic anthrax. Toxicon (2008) 52:824-8.
  2. CODOLO G, AMEDEI A, STEERE AC, PAPINUTTO E, CAPPON A, POLENGHI A, BENAGIANO M, PACCANI SR, SAMBRI V, DEL PRETE G, BALDARI CT, ZANOTII G, MONTECUCCO C, D’ELIOS MM, DE BERNARD M. Borrelia burgdorferi NapA-driven Th17 cell inflammation in Lyme arthritis. Arthritis Rheum. (2008) 58:3609-17.
  3. DAL MOLIN F., ZORNETTA I., PUHAR A., TONELLO F., ZACCOLO M. & MONTECUCCO C. cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. Biochem Biophys Res Commun. (2008) 376:429-433
  4. RIGONI M., PAOLI M., MILANESI E., CACCIN P., RASOLA A., BERNARDI P. & MONTECUCCO C. Snake phospholipase A2 neurotoxins enter neurons, bind specifically to mitochondria and open their transition pores. J Biol Chem. (2008) 283:34013-20.
  5. FASANELLA,A., TONELLO, F., GAROFALO G., MURARO L., CARATTOLI A., ADONE R. & MONTECUCCO C. Protective activity and immunogenicity of two recombinant anthrax vaccines for veterinary use. Vaccine (2008) 26:5684-5688.
  6. ROSSI PACCANI, S., DAL MOLIN F., BENAGIANO M., LADANT D., D’ELIOS M.M., MONTECUCCO, C. & BALDARI, T.C. Suppression of T lymphocyte activation and chemotaxis by the Adenylate Cyclase toxin of Bordetella pertussis.. Infect. & Immun. (2008) 76:2822-2832.
  7. ARCIDIACONO D., ODOM S., FROSSI B., RIVERA J., PACCANI S.R., BALDARI T.C., PUCILLO C., MONTECUCCO C. & DE BERNARD M. The Vibrio cholerae cytolysin (VCC) promotes activation of mast cell (T helper 2) cytokine production. Cell Microbiol. (2008) 10:899-907.
  8. CENDRON L., TASCA E., SERAGLIO T., SEYDEL A., ANGELINI A., BATTISTUTTA R., MONTECUCCO C. & ZANOTTI G. The crystal structure of CagS from Helicobacter pylori pathogenicity island. Proteins (2007) 69, 440-443.
  9. GINER D., LOPEZ I., NECO P., R0SSETTO O., MONTECUCCO C. & GUTIERREZ L.M. Glycogen synthase kinase 3 activation is essential for the snake phospholipase A2 neurotoxin-induced secretion in chromaffin cells. Eur J Neurosci. (2007) 25, 2341-2348.
  10. VERDERIO C., GRUMELLI C., RAITERI L., COCO S., PALUZZI S., CACCIN P., ROSSETTO O., MONTECUCCO C. & MATTEOLI M. Traffic of botulinum toxins A and E in excitatory and inhibitory neurons. Traffic (2007) 8, 142-153.
  11. CALEO M., RESTANI L., GIANFRANCESCHI L., COSTANTINI L., ROSSI C., ROSSETTO O., MONTECUCCO C. & MAFFEI L. Unilateral silencing of virtual cortex via botulinum neurotoxin affects cortical development in both hemispheres. J. Neurosci. (2007) 27, 4530-4540.


  1. MORBIATO L., CARLI L., JOHNSON E. A.,MONTECUCCO C., MOLGO J. & ROSSETTO O. Neuromuscular paralysis and recovery in mice injected with botulinum neurotoxins A and C, Eur. J. Neurosci. (2007) 25, 2697-704.


  1. POLENGHI A., BOSSI F., FISCHETTI F., DURIGUTTO P., CABRELLE A., TAMASSI N., CASSATELLA M.A., MONTECUCCO C., TEDESCO F., DE BERNARD M. The Neutrophil-Activating Protein of Helicobacter pylori crosses endothelia to promote neutrophil adhesion in vivo. J. Immunol. (2007) 178, 1312-1320.
  2. RIGONI M., PIZZO P., SCHIAVO G., WESTON A.E., ZATTI G., CACCIN P., ROSSETTO O., POZZAN T. and MONTECUCCO C. Calcium Influx and Mitochondrial Alterations at Synapses Exposed to Snake Neurotoxins or their Phospholipid Hydrolysis Products. J. Biol. Chem. (2007) 282, 11238-11245
  3. MEGIGHIAN A., RIGONI M., CACCIN P., ZORDAN M.A. and MONTECUCCO C. A lysolecithin/fatty acid mixture promotes and then blocks neurotransmitter release at the Drosophila melanogaster larval neuromuscular junction. Neurosci. Lett. (2007)  415, 6-11.
  4. GENISSET C., PUHAR A., CALORE F., DE BERNARD M., DELL’ANTONE P. and MONTECUCCO C. The concerted action of the Helicobacter pylori cytotoxin VacA and of the v-ATPase proton pump induces swelling of isolated endosomes. Cell. Microbiol. (2007) 9, 1481-1490.
  5. CACCIN P., RIGONI M., BISCEGLIE A., ROSSETTO O. & MONTECUCCO C.. Reversible Skeletal Neuromuscular Paralysis induced by different Lysophospholipids. FEBS Lett (2006) 580, 6317-6321.
  6. DAL MOLIN F., TONELLO F., LADANT D., ZORNETTA I, ZAMPARO I., DI BENEDETTO G.,  ZACCOLO M &  MONTECUCCO C. Cell Entry and cAMP Imaging of Anthrax Edema Toxin. EMBO J. (2006)  25, 5405-5413.
  7. ROSSI PACCANI, S., TONELLO, F., PATRUSSI L., CAPITANI N., SIMONATO M., MONTECUCCO, C§. & BALDARI, T.C§. Anthrax toxins inhibit immune cell chemotaxis by perturbing chemokine receptor signaling. Cell. Microbiol. (2007)  9, 924-929
  8. THUMIGER A., POLENGHI A., PAPINUTTO E., BATTISTUTTA R., MONTECUCCO C. & ZANOTTI G. Crystal structure of antigen TpF1 from Treponema pallidum. Proteins. (2006) 62, 827-830.
  9. BARBUTI G., MOSCHIONI M., CENSINI S., COVACCI A., MONTECUCCO C. & MONTEMURRO P. Streptococcus pneumoniae induces mast cell degranulation. Int. J. Med. Microbiol. (2006) 296, 325-329.
  10. LUVISETTO S., MARINELLI S., LUCCHETTI F., MARCHI F., COBIANCHI S., ROSSETTO O., MONTECUCCO C. & PAVONE F. Botulinum neurotoxins and formalin-induced pain: central versus peripheral effects in mice. Brain Res. (2006) 1082, 124-131.
  11. COUTURIER M.R., TASCA E., MONTECUCCO, C. & STEIN M. Interaction with CagF Is Required for Translocation of CagA into the Host via the Helicobacter pylori Type IV Secretion System. Infect Immun. (2006) 74, 273-281.
  12. ROMANELLO V. MARCACCI M., DAL MOLIN F., MOSCHIONI M., CENSINI S., COVACCIA. BARITUSSIO A.G., MONTECUCCO C. TONELLO F. Cloning, expression, purification, and characterization of Streptococcus pneumoniae IgA1 protease. Protein Expr. Purif. (2006) 45, 142-149.
  13. RIGONI M., CACCIN P., GSCHMEISSNER. S., KOSTER G., POSTLE A.D., ROSSETTO O., SCHIAVO G. & MONTECUCCO C.. Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures. Science (2005) 310, 1678-1680.

Perspective: Zimmerberg J, Chernomordik LV. Neuroscience. Synaptic membranes bend to the will of a neurotoxin. Science. 2005 Dec 9;310(5754):1626-7.

  1. PANTANO S. & MONTECUCCO C. A molecular model of the Vibrio cholerae cytolysin transmembrane pore. Toxicon (2005) 47, 35-40.
  2. TOURNIER J.N., QUESNEL-HELLMANN A., MATHIEU J., MONTECUCCO, C., TANG W,J., MOCK M., VIDAL D.R. & GOOSSENS .PL. Anthrax edema toxin cooperates with lethal toxin to impair cytokine secretion during infection of dendritic cells. J Immunol. (2005) 174, 4934-4941.
  3. BONANOMI D., PENNUTO M., RIGONI M., ROSSETTO O., MONTECUCCO C. & VALTORTA F. Taipoxin induces synaptic vesicle exocytosis and disrupts the interaction of synaptophysin I with VAMP2. Mol. Pharmacol. (2005) 67, 1901-1918.
  4. COSTANTINI L., BOZZI Y., RICHICHI C., VIEGI A., ANTONUCCI F., FUNICELLO M., GOBBI M., MENNINI T., ROSSETTO O., MONTECUCCO C., MAFFEI L., VEZZANI A. & CALEO M. Antiepileptic effects of botulinum neurotoxin E. J. Neurosci. (2005) 25, 1943-1951.
  5. COSTANTINI L., BOZZI Y., RICHICHI C., VIEGI A., ANTONUCCI F., FUNICELLO M., GOBBI M., MENNINI T., ROSSETTO O., MONTECUCCO C., MAFFEI L., VEZZANI A. & CALEO M. Antiepileptic effects of botulinum neurotoxin E. J. Neurosci. (2005) 25, 1943-1951.
  6. ROSSI PACCANI, S., TONELLO, F., GHITTONI, R., NATALE, M., MURARO L., D’ELIOS M.M., TANG, W.J, MONTECUCCO, C. & BALDARI, T.C. Anthrax Toxins suppress T-lymphocyte activation by disrupting receptor signalling. J. Exp. Med. (2005) 201, 325-331.

Highlighted by: J. Galan in JEM 201:

  1. de BERNARD, M., CAPPON, A. PANCOTTO, L., DEL GIUDICE, G. & MONTECUCCO, C. The Helicobacter pylori VacA cytotoxin induces intracellular calcium oscillations and secretion in mast cells. Cell. Microbiol. (2005) 7, 191-198.
  2. ELEOPRA, R., TUGNOLI, V., QUATRALE, R., ROSSETTO, O. & A., MONTECUCCO, C. Different types of botulinum toxin in humans. Mov. Disord. (2004) 19, S53-S59.
  3. RIGONI, M., SCHIAVO G., WESTON A.E., CACCIN, P., ALLEGRINI F., PENNUTO M, VALTORTA F., MONTECUCCO, C. & ROSSETTO, O. Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles. J. Cell Sci. (2004) 117, 3561-3570
  4. LUVISETTO S., MARINELLI S., ROSSETTO O., MONTECUCCO C. & PAVONE F. Central injection of botulinum neurotoxins: behavioural effects in mice. Behavioural Pharmacol. (2004) 15, 233-240
  5. PUHAR, A., JOHNSON, E.A., ROSSETTO O. & MONTECUCCO, C. Comparison of the pH-induced conformational change of the different clostridial neurotoxins. Biochem. Biophys. Res. Commun. (2004) 319, 9-14.
  6. DALL’AICA, I., DONA’, M., TONELLO, F., PIRIS, A., MOCK, M., MONTECUCCO, C. & GARBISA, S. Potent inhibitors of anthrax lethal factor from green tea. EMBO Reports (2004) 5, 418-422.
  7. TONELLO, F., NALETTO, L., ROMANELLO, V., DAL MOLIN, F. & MONTECUCCO, C. Tyrosine-728 and glutamic-735 are essential for the metalloproteolytic activity of the lethal factor of Bacillus anthracis. Biochem. Biophys. Res. Commun. (2004) 313, 496-502.
  8. TONELLO, F., ASCENZI, P. & MONTECUCCO, C. The metalloproteolytic activity of the anthrax lethal factor is substrate-inhibited. J. Biol. Chem. (2003) 278, 40075-40078.
  9. CACCIN, P., ROSSETTO, O., RIGONI, M., SCHIAVO, G. & MONTECUCCO, C.. VAMP/synaptobrevin cleavage by tetanus and botulinum neurotoxins is strongly enhanced by acidic liposomes. FEBS Lett. (2003) 532, 132-136.
  10. NISHIOKA, H., BAESSO I., SEMENZATO, G., TRENTIN L., RAPPUOLI, R., DEL GIUDICE, G. & MONTECUCCO C. The neutrophil activating protein of Helicobacter pylori (HP-NAP) activates the MAPK pathway in human neutrophils. Eur. J. Immunol. (2003) 33, 840-849.
  11. NECO P., ROSSETTO, O., GIL A., MONTECUCCO, C. & GUTIERREZ, L. Taipoxin induces F-actin fragmentation and enhances release of catecholamines in bovine chromaffin cells. J. Neurochem. (2003) 85, 329-337.


  1. LUVISETTO, S., ROSSETTO, O., MONTECUCCO, C. & PAVONE, F. Toxicity of botulinum neurotoxins in central nervous system of mice. Toxicon (2003) 41, 475-481.
  2. SCAPINI, P., NARDELLI, B., NADALI, G., CALZETTI, F., PIZZOLO, G., MONTECUCCO, C. & CASSATELLA, M. G-CSF-stimulated neutrophils are a prominent source of functional BLyS. J. Exp. Med. (2003) 197, 840-849.
  3. TUGNOLI, V., MARCHESE RAGONA, R., ELEOPRA, R., QUATRALE, R., CAPONE, J. G., PASTORE, A., MONTECUCCO, C. & DE GRANDIS, E. The role of gustatory flushing in Frey’s syndrome and its treatment with botulinum toxin type A. Clin Auton Res (2002) 3, 174-178


  1. ZANOTTI, G., PAPINUTTO, E., DUNDON, W.G., BATTISTUTTA, R., SEVESO, M., DEL GIUDICE, G. RAPPUOLI, R., & MONTECUCCO, C. Structure of the Neutrophil-activating protein from Helicobacter pylori. J. Mol. Biol. (2002) 323, 125-130.
  2. SEYDEL, A., TASCA, E., BERTI, D., RAPPUOLI, R., DEL GIUDICE, G. & MONTECUCCO, C. Characterization and immunogenicity of the CagF protein of the cag pathogenicity island of Helicobacter pylori. Infect. Immun. (2002) 70, 6468-6470.
  3. TONELLO, F., SEVESO, M., MARIN, O., MOCK, M. & MONTECUCCO, C. Pharmacology: Screening inhibitors of anthrax lethal factor. Nature (2002) 418, 386.
  4. MOSCHIONI, M., TOMBOLA, F., DE BERNARD, M., COEHLO, A., ZITZER, A., ZORATTI, M. & MONTECUCCO, C. The Vibrio cholerae hemolysin anion channel is required for cell vacuolation and death. Cell. Microbiol. (2002) 4, 397-409.
  5. ELEOPRA, R., TUGNOLI, V., QUATRALE, R., GASTALDO, E., ROSSETTO, O., DE GRANDIS, D. & MONTECUCCO, C. Botulinum neurotoxins serotype A and C do not affect motor units survival in humans: an electrophysiological study by motor unit counting. Clin. Neurophysiol. (2002) 113, 1258-64.
  6. PAPINUTTO, E., DUNDON, W. G., PITULIS, N., BATTISTUTTA, R., MONTECUCCO, C. & ZANOTTI, G. Structure of two iron-binding proteins from Bacillus anthracis . J. Biol. Chem. (2002) 277, 15093-15098.
  7. MONTEMURRO, P., NISHIOKA, H., DUNDON W.G., DE BERNARD, M., DEL GIUDICE, G., RAPPUOLI, R. & MONTECUCCO, C. The Neutrophil Activating Protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells. Eur. J. Immunol. (2002) 32, 671-676.
  8. de BERNARD, M., MOSCHIONI, M., HABERMANN, A., GRIFFITHS, G. & MONTECUCCO, C. Cell vacuolization induced by Helicobacter pylori VacA cytotoxin does not depend on late endosomal SNAREs. Cell. Microbiol., (2002) 4, 11-18.
  9. RIGONI, M., CACCIN, P., JOHNSON, E., MONTECUCCO C. & ROSSETTO, O. Site-directed mutagenesis identifies active-site residues of the light chain of botulinum neurotoxin type A. Biochem. Biophys. Res Commun. (2001) 288, 1231-1237.
  10. TOMBOLA, F., PAGLIACCIA, C., CAMPELLO, S., TELFORD, J.L., MONTECUCCO, C., PAPINI, E. & ZORATTI, M. How the loop and middle regions influence the properties of Helicobacter pylori VacA Channels. Biophys. J. (2001) 81, 3204.3215.
  11. DE BELLIS, L., PAPINI, E., MONTECUCCO, C. & CURCI, S. Helicobacter pylori cytotoxin VacA increases alkaline secretion in gastric epithelial cells. Am. J. Physiol. (2001) 281, G1440-1448.
  12. ROSSETTO, O., CACCIN, P., RIGONI, M., TONELLO, F., BORTOLETTO, N., STEVENS R.C. & MONTECUCCO, C. Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity. Toxicon (2001) 39, 1151-1159.
  13. DUNDON, W.G., POLENGHI, A., DEL GIUDICE, G., RAPPUOLI, R. & MONTECUCCO, C. Neutrophil-activating protein (HP-NAP) versus ferritin (Pfr): comparison of synthesis in Helicobacter pylori. FEMS Microbiol. Lett. (2001) 199, 143-149.
  14. TUGNOLI, V., MARCHESE RAGONA, R., DE GRANDIS, D., ELEOPRA, R. & MONTECUCCO, C. The treatment of Frey syndrome with botulinum toxin type F. Arch. Otolaryng. Head & Neck Surg. (2001) 127, 339-340.
  15. MONTEMURRO, P., BARBUTI, G., DUNDON, W.G., MONTECUCCO, C., DEL GIUDICE, G., RAPPUOLI, R., COLUCCI, M., SEMERARO, N., DE RINALDIS, P. & PAPINI, E. HP-NAP of Helicobacter pylori alters the coagulation-fibrinolysis balance of human blood mononuclear cells by stimulating the expression of Tissue Factor and PAI-2. J. Infect. Dis. (2001) 183, 1055-1062
  16. PELLIZZARI, R., GUIDI-RONTANI, C., VITALE, G., MOCK, M. & MONTECUCCO, C. Lethal factor of Bacillus anthracis cleaves the N-terminus of MAPKKs: analysis of the intracellular consequences in macrophages. Int. J. Med. Microbiol. (2000), 290, 421-427.
  17. VITALE, G., BERNARDI, L., NAPOLETANI, G., MOCK, M. & MONTECUCCO, C. Susceptibility of MAP kinase kinases to anthrax lethal factor proteolysis: target specificity and cleavage sites. Biochem. J. (2000) 352, 739-745.
  18. BERNARDI, L., VITALE, G., MONTECUCCO, C. & MUSACCHIO, A. Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor. Acta Crystallographica, (2000) D56, 1449-1451.
  19. HERREROS, J., LALLI, G., MONTECUCCO, C. & SCHIAVO, G. Tetanus toxin fragment C binds to a protein present in neuronal cell lines and motoneurons. J. Neurochem, (2000) 74 , 1941-1950
  20. de BERNARD, M., MOSCHIONI, M., NAPOLITANI , G., RAPPUOLI, R. & MONTECUCCO, C. The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein. EMBO J. (2000) 19, 48-56.
  21. SATIN, B., DEL GIUDICE, G., DELLA BIANCA, V., G., DUSI, S., LAUDANNA, C., TONELLO, F., KELLEHER, D., RAPPUOLI, R., MONTECUCCO, C. & ROSSI, F. The Neutrophil Activating Protein (HP-NAP) of Helicobacter pylori is a protective antigen and a major virulence factor J. Exp. Med, (2000) 191, 1467-1476.
  22. PELLIZZARI, R., GUIDI-RONTANI, C., VITALE, G., MOCK, M. & MONTECUCCO, C. Anthrax lethal factor cleaves MEKK3 in macrophages and inhibits the LPS/IFNgamma-induced release of NO and TNFalpha. FEBS Lett. (1999) 462, 199-204.
  23. VERDERIO, C., COCO, S., BACCI, A., ROSSETTO, O., DE CAMILLI, P., MONTECUCCO, C. and MATTEOLI, M.. Tetanus toxin blocks the exocytosis of synaptic vesicles clustered at synapses but not of synaptic vesicles in isolated axons. J. Neuroscience (1999) 19, 6723-6732.
  24. SZABO, I., BRUTSCHE; S., TOMBOLA, F.,, MOSCHIONI, M., SATIN, B., TELFORD, J.L., RAPPUOLI, R., MONTECUCCO, C., PAPINI, E. & ZORATTI, M. . Formation of anion-selective channels in the cell plasma membrane by the toxin VacA of Helicobacter pylori is required for its biological activity. EMBO J. (1999) 18, 5517-5527
  25. TOMBOLA, F., OREGNA, F., BRUTSCHE, S., SZABO, RAPPUOLI, R., MONTECUCCO, C., PAPINI, E. & ZORATTI, M. Inhibition of the vacuolating and anion activities of the VacA toxin of Helicobacter pylori. FEBS Lett. (1999) 460, 221-225.
  26. TONELLO, F., DUNDON, W.G., SATIN, B., MOLINARI, M., TOGNON, G., GRANDI, G., DEL GIUDICE, G., RAPPUOLI, R. & MONTECUCCO. The Helicobacter pylori neutrophil activating protein is an iron binding protein with dodecameric structure. Mol. Microbiol. (1999) 34:238-247.
  27. LALLI, G., HERREROS, J., OSBORNE, S.L., MONTECUCCO, C., ROSSETTO, O. & SCHIAVO, G. Functional characterization of tetanus and botulinum neurotoxins binding domains. J. Cell Sci. (1999) 112, 2715-2724
  28. WASHBOURNE, P., BORTOLETTO, N., GRAHAM, M.E., WILSON, M.C., BURGOYNE, R.D. & MONTECUCCO, C. Botulinum Neurotoxin E insensitive mutants of SNAP-25 fail to bind VAMP, but support exocytosis. J. Neurochem. (1999) 73, 2424-2433
  29. PELICIC, V., REYRAT, J.-M., SARTORI, L.,PAGLIACCIA, C., RaAPPUOLI, R., TELFORD, J.L. MONTECUCCO, C. & PAPINI, E. Helicobacter pylori VacA cytotoxin associated with bacteria increases epithelial permeability independently of its vacuolating activity. Microbiology (1999) 145, 2043-2050.
  30. VERDERIO, C., COCO, S., ROSSETTO, O., MONTECUCCO, C. and MATTEOLI, M.. Internalization and proteolytic action of botulinum toxins in CNS neurons and astrocytes. J. Neurochem. (1999) 73, 372-379.
  31. TOMBOLA, F., CARLESSO, C., SZABO, I., REYRAT, J.M., TELFORD, J.L., RAPPUOLI, R., MONTECUCCO, C., PAPINI, E. & ZORATTI, M. Helicobacter pylori vacuolating toxin forms anion-selective channels in planar lipid bilayers. Biophys. J. (1999) 76, 1401-1409
  32. TONELLO, F., PELLIZZARI, R., PASQUALATO, S., GRANDI, G., PEGGION, E. & MONTECUCCO. Recombinant and truncated tetanus neurotoxin light chain: Cloning, Expression, Purification and Proteolytic Activity. Prot. Expres. Purif. (1999) 15, 221-227
  33. BENJELLOUN-TOUIMI, Z., TAHAR, M.S., MONTECUCCO, C., SANSONETTI, P.J. & PARSOT, C. SepA, the 110 kDa protein secreted by Shigella flexneri: structure and proteolytic activity. Microbiology (1998) 144, 1815-1822.
  34. ELEOPRA, R., TUGNOLI, V., ROSSETTO, O., DE GRANDIS, D. & MONTECUCCO, C. Different time courses of recovery after poisoning with botulinum neurotoxin serotypes A and E in humans. Neurosci. Lett. (1998) 256, 135-138.
  35. DE BERNARD, M., MOSCHIONI, M., PAPINI, E., TELFORD, J.L., RAPPUOLI, R. & MONTECUCCO, C. TPA and butyrate increase cell sensitivity to the vacuolating toxin of Helicobacter pylori. FEBS Lett. (1998) 436, 218-222.
  36. PELLIZZARI, R., ROSSETTO, O. , WASHBOURNE, P., TONELLO, F., NICOTERA, P. & MONTECUCCO, C. In vitro biological activity and toxicity of tetanus and botulinum neurotoxins. Toxicol. Lett. (1998) 103, 191-197.
  37. DE BERNARD, M., BURRONI, D., PAPINI, E., RAPPUOLI, R., TELFORD, J. L. & MONTECUCCO, C. Identification of the Helicobacter pylori VacA toxin active domain active in the cell cytosol. Infect. Immun. (1998) 66, 6014-6016
  38. VITALE, G., PELLIZZARI, R., RECCHI, C., NAPOLITANI, G., MOCK, M. & MONTECUCCO, C. Anthrax Lethal factor cleaves the N-terminus of MAPKKs and induces Tyrosine/Threonine phosphorylation of MAPKs in cultured macrophages. Biochem. Biophys. Res. Commun. (1998) 248, 706-711.
  39. DE BERNARD, M., MOSCHIONI, M., PAPINI, E., TELFORD, J., RAPPUOLI, R. & MONTECUCCO, C. Cell vacuolization induced by Helicobacter pylori VacA toxin: cell line sensitivity and quantitative extimation. Toxicol. Lett. (1998) 99, 109-115.
  40. MOLINARI, M., GALLI, C., DE BERNARD, M., NORAIS, N., RUYSSCHAERT, J.M., RAPPUOLI, R. and MONTECUCCO, C. The acid activation of Helicobacter pylori toxin VacA: structural and membrane binding studies. Biochem. Biophys. Res. Commun. (1998) 248, 334-340.
  41. PAPINI, E., SATIN, B., NORAIS, N., DE BERNARD, M., TELFORD, J.L., RAPPUOLI, R. and MONTECUCCO, C. Selective increase of the permeability of polarised epithelial cell monolayers by Helicobacter pylori vacuolating toxin. J. Clin. Inv. (1998) 102, 813-820.
  42. MOLINARI, M., SALIO, M., GALLI, C., NORAIS, N., RAPPUOLI, R., LANZAVECCHIA, A. & MONTECUCCO, C. Selective inhibition of Li-dependent antigen presentation by Helicobacter pylori toxin VacA. J. Exp. Med. (1998) 187, 135-140.
  43. ELEOPRA, R., TUGNOLI, V., ROSSETTO, O., MONTECUCCO, C. & DE GRANDIS, D. Botulinum neurotoxin serotype C: a novel effective botulinum toxin therapy in human. Neurosci. Lett. (1997) 224, 91-94.
  44. WASHBOURNE, P., PELLIZZARI, R., BALDINI, G., WILSON, M.C. & MONTECUCCO, C. Botulinum neurotoxin types A and E require the SNARE motif in SNAP-25 for proteolysis. FEBS Lett. (1997) 418, 1-5.
  45. DE BERNARD, M., ARICO’, B., PAPINI, E., RIZZUTO, R., GRANDI, C., RAPPUOLI, R. & MONTECUCCO, C. Helicobacter pylori toxin VacA. induces vacuole formation by acting in the cell cytosol. Mol. Microbiol. (1997) 26, 665-674.
  46. MOLINARI, M., GALLI, C., NORAIS, N., TELFORD, J., RAPPUOLI, R., LUZIO, J.P.. & MONTECUCCO, C. Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers. J. Biol. Chem. (1997) 272, 25339-25344.
  47. SATIN, B., NORAIS, N., TELFORD, J., RAPPUOLI, R., MURGIA, M., MONTECUCCO, C. & PAPINI, E. Effect of Helicobacter pylori vacuolating toxin on maturation and extracellular release of procathepsin D and epidermal growth factor degradation. J. Biol. Chem. (1997) 272, 25022-25028.
  48. LEIST, M., FAVA, E., MONTECUCCO, C. and NICOTERA, P. Peroxynitrite and NO-donors induce neuronal apoptosis by eliciting autocrine excitotoxicity. Eur. J. Neurosci. (1997) 9, 1488-1498
  49. PELLIZZARI, R., MASON, S., SHONE, C.C. & MONTECUCCO, C. The interaction of synaptic vesicle-associated membrane protein synaptobrevin with botulinum neurotoxins D and F. FEBS. Lett. (1997) 409, 339-342.
  50. TONELLO, F., SCHIAVO, G. & MONTECUCCO, C. Metal-substituted tetanus neurotoxin. Biochem. J. (1997) 322, 507-510


  1. PAPINI, E., SATIN, B., BUCCI, C., DE BERNARD, M., TELFORD, J., MANETTI, R., RAPPUOLI, R. ZERIAL, M. and MONTECUCCO, C. The small GTP-binding protein rab7 is essential for cellular vacuolation induced by Helicobacter pylori cytotoxin. EMBO J. (1997) 16, 15-24
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  3. MATTEOLI, M., VERDERIO, C., ROSSETTO, O., IEZZI, N., COCO, S., SCHIAVO and MONTECUCCO, C. Synaptic vesicle endocytosis mediates the entry of tetanus neurotoxin into hyppocampal neurons. Proc. Natl. Acad. Sci. USA (1996) 93, 13310-13315.
  4. PELLIZZARI, R., ROSSETTO, O., LOZZI, L., GIOVEDI, S., JOHNSON, E., SHONE, CC. and MONTECUCCO, C. Structural determinants of the specificity for VAMP/synaptobrevin of tetanus and botulinum type B and G neurotoxins. J. Biol. Chem. (1996) 271, 20353-20358

105.MENARD, A., ALTENDORF, K., BREVES, D., MOCK, M. and MONTECUCCO, C. The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis lethal factor. FEBS Lett. (1996) 386, 161-164

  1. WITCOME, M., ROSSETTO, O., MONTECUCCO, C. and SHONE, C.C. Substrate residues distal to the cleavage site of botulinum type B neurotoxin play a role in determining the specificity of its endopeptidase activity. FEBS Lett. (1996) 386, 133-136
  2. WILLIAMSON, L.C., HALPERN, J.L., MONTECUCCO, C., BROWN, J.E. and NEALE, E.A. Clostridial neurotoxins and substrate proteolysis in intact neurons: botulinum neurotoxin C acts on SNAP-25. J. Biol. Chem. (1996) 271, 7694-7699.
  3. OSEN-SAND, A., STAPLE, J.K., NALDI, E., SCHIAVO, G., ROSSETTO, O., PETITPIERRE, S., MALGAROLI, A., MONTECUCCO, C. and CATSICAS, S. Common and distinct fusion-proteins in axonal growth and transmitter release. J. Comp. Neurol. (1996) 367, 222-234.
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  5. MORANTE, S., FURENLID, L., SCHIAVO, G., TONELLO, ZWILLING, R. & MONTECUCCO, C. A X-ray absorption spectroscopy study of zinc coordination in tetanus neurotoxin, astacin, thermolysin and alkaline protease. Eur. J. Biochem. (1996) 235, 606-612.
  6. MOLL G., PAPINI E., COLONNA R., BURRONI D., TELFORD J., RAPPUOLI R. & MONTECUCCO C. Lipid interaction of the 37 kDa and 58 kDa fragments of the Helicobacter pylori cytotoxin. Eur. J. Biochem. (1996) 234, 947-952
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  8. MENARD A., MOCK M. & MONTECUCCO, C. Similarities among the lethal factor of Bacillus anthracis and leukotriene A4 hydrolase. Mol. Microbiol. (1996) 18, 991-992
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  10. DE BERNARD M., PAPINI E., DE FILIPPIS E., GOTTARDI E., TELFORD J., MANETTI M., FONTANA A., RAPPUOLI R. & MONTECUCCO C. Low pH activates the Vacuolating Toxin of Helicobacter pylori which becomes acid and pepsin resistant. J. Biol. Chem. (1995) 270, 23937-23940
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  17. SADOUL K., LANG J., MONTECUCCO C., WELLER U., REGAZZI R., CATSICAS S., WOLLHEIM C.B. & HALBAN, P.A. SNAP-25 is expressed in islets of Langerhans and is involved in insulin release. J. Cell Biol. (1995) 128, 1019-1028.
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  19. SCHIAVO G., SHONE C.C., BENNETT M.K., SCHELLER R.H. & MONTECUCCO C. Botulinum neurotoxin C cleaves a single peptide bond within the carboxyl-terminal region of different syntaxin isoforms. J. Biol. Chem. (1995) 270, 10566-10570
  20. KOCHI S.K., SCHIAVO G., MOCK M. & MONTECUCCO C. Zinc content of the Bacillus anthracis lethal factor. FEMS Microbiol. Lett. (1994) 124, 343-348.
  21. ROSSETTO O., SCHIAVO G., MONTECUCCO C., POULAIN B., DELOYE F., LOZZI L. & SHONE C.C. SNARE motif and neurotoxin binding. Nature (1994) 372, 415-416.
  22. PAPINI E., DE BERNARD M., MILIA E., BUGNOLI M., ZERIAL M., RAPPUOLI R. & MONTECUCCO C. Cellular vacuoles induced by Helicobacter pylori originate from late endosomal compartments. Proc. Natl. Acad. Sci. U.S.A. (1994) 91, 9720-9724
  23. SCHIAVO G., MALIZIO C., TRIMBLE W.S., POLVERINO DE LAURETO P., MILAN G., SUGIYAMA H., JOHNSON E.A. & MONTECUCCO C. Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond. J. Biol. Chem. (1994) 269, 20213-20216
  24. TELFORD J.L., GHIARA P., DELL’ORCO M., COMANDUCCI M., BURRONI D., BUGNOLI M., TECCE M.F., CENSINI S., COVACCI A., XIANG Z., PAPINI E., MONTECUCCO C. & RAPPUOLI R. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med. (1994) 179, 1653-1658
  25. PATARNELLO T., BARGELLONI L., ROSSETTO, O., SCHIAVO G. & MONTECUCCO C. Neurotransmission and secretion. Nature (1993) 364, 581-582
  26. SCHIAVO G., SANTUCCI A., DASGUPTA B.R., METHA P.P., JONTES J., BENFENATI F., WILSON M.C. & MONTECUCCO C. Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds. F.E.B.S. Lett. (1993) 335, 99-103
  27. SCHIAVO G., ROSSETTO O., CATSICAS S., POLVERINO DE LAURETO P., DASGUPTA B.R., BENFENATI F. & MONTECUCCO C. Identification of the nerve-terminal targets of botulinum neurotoxins serotypes A, D and E. J. Biol. Chem. (1993) 268, 23784-23787with highlights in Nature News & Views by W.B. Huttner. Snappy exocytotoxins. Nature (1993) 365, 104-105
  28. PAPINI E., DE BERNARD M., BUGNOLI M., MILIA E., RAPPUOLI R. & MONTECUCCO C. Cell vacuolization induced by Helicobacter pylori: inhibition by bafilomycins A1, B1, C1 and D. F.E.M.S. Microbiol. Lett. (1993) 113, 155-160
  29. POULAIN B., ROSSETTO O., DELOYE F., SCHIAVO G., TAUC L. & MONTECUCCO C. Antibodies against rat-brain VAMP/synaptobrevin prevent inhibition of acetylcholine release by tetanus toxin or botulinum neurotoxin type B. J. Neurochem.(1993) 61, 1175-1178
  30. SCHIAVO G., SHONE C.C., ROSSETTO O., ALEXANDER F.C.G. & MONTECUCCO C. Botulinum neurotoxin serotype F is a zinc endopeptidase specific for VAMP/synaptobrevin. J. Biol. Chem. (1993) 268, 11516-11519.
  31. PAPINI E., BUGNOLI M., DE BERNARD B., FIGURA N., RAPPUOLI R. & MONTECUCCO C. Bafilomycin A1 inhibits Helicobacter pylori induced vacuolization of HeLa cells. Mol. Microbiol. (1993) 7, 323-327.
  32. PAPINI E., CABRINI G. & MONTECUCCO C. Cystic fibrosis cells sensitivity to diphtheria toxin. Toxicon (1993) 31, 359-362.
  33. PAPINI E., RAPPUOLI R., MURGIA M. & MONTECUCCO C. Cell penetration of diphtheria toxin: reduction of the interchain disulfide bridge is the rate limiting step of translocation in the cytosol. J. Biol. Chem. (1993) 268, 1567-1574.
  34. SCHIAVO G., BENFENATI F., POULAIN B., ROSSETTO O., POLVERINO DE LAURETO P., DAS GUPTA B.R. & MONTECUCCO C. Tetanus and botulinum B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin. Nature (1992) 359, 832-835. With highlights in Nature News and Views by D. Cutler. Cell Biology: Progress by Poisoning. Nature. (1992) 359, 773.
  35. SCHIAVO G., POULAIN B., ROSSETTO O., BENFENATI F., TAUC L. & MONTECUCCO C. Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release depends on zinc. EMBO J. (1992) 11, 3577-3583.
  36. ROSSETTO O., SCHIAVO G., POLVERINO DE LAURETO P., FABBIANI S. & MONTECUCCO C. Surface topography of histidine residues of tetanus toxin probed by immobilized-metal-ion affinity chromatography. Biochem. J. (1992) 285, 9-12.
  37. CALAPPI E., MASSERINI M., SCHIAVO G., MONTECUCCO C. & TETTAMANTI G. Lipid interaction of tetanus neurotoxin: a calorimetric and fluorescence spectroscopy study. FEBS Lett. (1992), 309, 107-110.
  38. SCHIAVO G., ROSSETTO O., SANTUCCI A., DASGUPTA B.R. & MONTECUCCO C. Botulinum neurotoxins A, B and E are zinc proteins. J. Biol. Chem. (1992) 267, 23479-23483.
  39. MONTECUCCO C., PAPINI E., SCHIAVO G., PADOVAN E. & ROSSETTO R. Ion channel and membrane translocation of diphtheria toxin. F.E.M.S. Microbiol. Immunol. (1992) 105, 101-111.
  40. SCHIAVO G., FERRARI G., ROSSETTO O. & MONTECUCCO C. Tetanus toxin receptor: specific cross-linking of tetanus toxin to a protein of NGF-differentiated PC12 cells. F.E.B.S Lett. (1991) 290, 227-230.
  41. SCHIAVO G., DEMEL R. & MONTECUCCO C. On the role of polysialogangliosides as tetanus toxin receptors: a study with lipid monolayers. Eur. J. Biochem. (1991) 199, 705-711.
  42. DEMEL R., SCHIAVO G., DE KRUIJFF B. & MONTECUCCO C. Lipid interaction of diphtheria toxin and mutants: a study with phospholipid and protein monolayers. Eur. J. Biochem. (1991) 197, 481-486.
  43. DOMENIGHINI M., MONTECUCCO C., RIPKA W.C. & RAPPUOLI R. Computer modelling of the NAD binding site of ADP-ribosylating toxins: active-site structure and mechanism of NAD binding. Mol. Microbiol.  (1991) 5, 23-31.
  44. PADOVAN E., PAPINI E., RAPPUOLI R. & MONTECUCCO C. Determination of diphtheria toxin neutralizing antibody titers with a cell protein synthesis inhibition assay. Med. Microbiol. Immunol. (1991) 180, 29-35.
  45. PAPINI E., SANTUCCI A., SCHIAVO G., DOMENIGHINI M., NERI P., RAPPUOLI R. & MONTECUCCO C. Tyr-65 is photolabeled by 8-azido-adenine and 8-azido-adenosine at the NAD binding site of diphtheria toxin. J. Biol. Chem. (1991) 266, 2494-2498.
  46. SCHIAVO G., PAPINI E., GENNA G. & MONTECUCCO C. An intact interchain disulfide bond is required for the neurotoxicity of tetanus toxin. Infect. Immun. (1990) 58, 4136-4141.


  1. PAPINI E., SCHIAVO G., RAPPUOLI R. & MONTECUCCO C. Histidine-21 is involved in diphtheria toxin NAD binding. Toxicon (1990) 28, 631-635.
  2. HARSHMAN S., BOQUET P., DUFLOT E., ALOUF J., MONTECUCCO C. & PAPINI E. Staphylococcal à-toxin: a study of membrane penetration and pore formation. J. Biol. Chem. (1989) 264, 14978-14984.
  3. PAPINI E., SCHIAVO G., SANDONA’ D., RAPPUOLI R. & MONTECUCCO C. Histidine-21 is at the NAD binding site of diphtheria toxin. J. Biol. Chem. (1989) 264, 12385-12388.
  4. MONTECUCCO C., SCHIAVO G. & DASGUPTA B.R. Effect of pH on the interaction of botulinum neurotoxins A, B and E with liposomes. Biochem. J. (1989) 259, 47-53.
  5. PAPINI E., SANDONA’ D., RAPPUOLI R. & MONTECUCCO C. On the membrane translocation of diphtheria toxin: at low pH the toxin induces ion channels on cells. EMBO J. (1988) 7, 3363-3369.
  6. XU S., CRAMER W.A., PETERSON A.A., HERMODSON M. & MONTECUCCO C. Dynamic properties of membrane proteins: reversible insertion into membrane vesicles of a colicin E1 channel-forming peptide. Proc. Natl. Acad. Sci. U.S.A. (1988) 85, 7531-7535.
  7. MONTECUCCO C., SCHIAVO G., GAO Z., BAUERLEIN E., BOQUET P. & DASGUPTA B.R. Interaction of botulinum and tetanus toxins with the lipid bilayer surface. Biochem. J. (1988) 251, 379-383
  8. MONTECUCCO C. Photoreactive lipids for the study of membrane-penetrating toxins. Methods Enzymol. (1988) 165, 347-357
  9. MULDERS J.W.M., VOORTER C.E.M., LAMERS C., de HAARD-HOCKMAN W.A., MONTECUCCO C., van de VEN W.J.M., BLOEMENDAL H. & de JONG W.W. MP17, a fiber-specific intrinsic membrane protein from mammalian eye lens. Curr. Eye Res. (1988) 7, 207-219
  10. BAIOCCHI M., PESCARMONA M., BRUSCHI M.L., MONTECUCCO C., SQUATRITI T. & TOMASI M. Selective extraction of hemagglutinin and matrix protein from Sendai virions by employing trifluoperazine as a detergent. F.E.B.S. Lett. (1988) 238, 171-174
  11. MONTECUCCO C. On the enzymatic activity of tetanus toxin. Toxicon (1987) 25, 1255-1257
  12. MONTECUCCO C., PAPINI E. & PITTON G. Does tetanus toxin have a sequence homology with the hemagglutinin of influenza virus? Toxicon (1987) 25, 911-912
  13. PAPINI E., COLONNA R., CUSINATO F., MONTECUCCO C., TOMASI M. & RAPPUOLI, R. Lipid interaction of diphtheria toxin and mutants with altered fragment B: Part I. Liposome aggregation and fusion. Eur. J. Biochem. (1987) 169, 629-635.
  14. PAPINI E., SCHIAVO G., TOMASI M., COLOMBATTI M., RAPPUOLI R. & MONTECUCCO C. Lipid interaction of diphtheria toxin and mutants with altered fragment B: Part II. Hydrophobic photolabelling and cell intoxication. Eur. J. Biochem. (1987) 169, 637-644
  15. PAPINI E., COLONNA R., SCHIAVO G., CUSINATO F., TOMASI M. & RAPPUOLI R. & MONTECUCCO C. Diphtheria toxin and its mutant crm 197 differ in their interaction with lipids. F.E.B.S. Lett. (1987) 215, 73-78
  16. MOSCUFO N., GALLINA A., SCHIAVO G., MONTECUCCO C. & TOMASI M. Multiple lipid interactions of Sendai virus fusogenic protein. J. Biol. Chem. (1987) 262, 11490-11496
  17. BISSON R., SCHIAVO G. & MONTECUCCO C. ATP induces conformational changes in mitochondrial cytochrome c oxidase: effect on the cytochrome c binding site. J. Biol. Chem. (1987) 262, 5592-5598
  18. MONTECUCCO C., SCHIAVO G., BACCI, B. & BISSON R. Isolation and characterization of cytochrome c oxidase from bird and fish heart mitochondria. Comp. Biochem. Physiol. (1987) 87B, 851-856
  19. VOLPE P., GUTWENIGER H.E. & MONTECUCCO C. Photolabeling of the integral proteins of skeletal muscle sarcoplasmic reticulum: comparison of junctional and nonjunctional membrane fractions. Arch. Biochem. Biophys. (1987) 253, 138-145.
  20. MONTECUCCO C. & SCHIAVO G. A photoactive phospholipid for the labelling of membrane components: 1-palmitoyl-2(p-benzoyl)benzoyl phosphatidylcholine. Biochem. J. (1986) 237, 309-312.
  21. DEGLI ESPOSTI M., AVITABILE E., BARILLI M., SCHIAVO G., MONTECUCCO C. and LENAZ G. Comparative biochemistry of the ubiquinol:cytochrome c oxidoreductase isolated from different heart mitochondria. Comp. Biochem. Physiol. (1986) 85, 543-552.
  22. ESCUYER V., BOQUET P., PERRIN D., MONTECUCCO C. & MOCK M. A pH induced increase in hydrophobicity as a possible step in the penetration of colicin E3 through bacterial membranes. J. Biol. Chem. (1986) 261, 10891-10898.
  23. MONTECUCCO C., TOMASI G., SCHIAVO G. & RAPPUOLI R. Hydrophobic photolabelling of pertussis toxin subunits interacting with lipids. F.E.B.S Lett. (1986) 194, 301-304.
  24. MONTECUCCO C., SCHIAVO G. & BISSON R. ATP binding to bovine heart cytochrome c oxidase: a photoaffinity labelling study. Biochem. J. (1986) 234, 241-243.
  25. MONTECUCCO C., SCHIAVO G., BRUNNER J., DUFLOT E., BOQUET P. & ROA M. Tetanus toxin is labeled with photoactivatable phospholipids at low pH. Biochemistry (1986) 25, 919-924.
  26. GUTWENIGER H. & MONTECUCCO C. Hydrophobic photolabelling of sarcoplasmic reticulum with 125I-TID. Int. J. Biochem. (1985) 17, 867-871
  27. GIRAUDAT J., MONTECUCCO C., BISSON R. & CHANGEUX J.P. Transmembrane topology of acetylcholine receptor subunits probed with photoreactive phospholipids. Biochemistry (1985) 24, 3121-3127
  28. BISSON R. & MONTECUCCO C. Helical packing in the hydrophobic sector of cytochrome c oxidase. J. Inorg. Biochem. (1985) 23, 177-182
  29. GUTWENIGER H.E. & MONTECUCCO C. Labelling of the integral proteins of sarcoplasmic-reticulum membranes. Biochem. J. (1984) 220, 613-616
  30. MONTECUCCO C., DABBENI-SALA., FRIEDL P. & GALANTE Y.M. Membrane topology of ATP synthase from bovine heart mitochondria and Escherichia coli. Eur. J. Biochem. (1983) 132, 189-194
  31. HOPPE J., MONTECUCCO C. & FRIEDL P. Labeling of subunit b of the ATP synthase from Escherichia coli with a photoreactive phospholipid analogue. J. Biol. Chem. (1983) 258, 2882-2885.
  32. BISSON R. & MONTECUCCO C. Different polypeptides of bovine heart cytochrome c oxidase are in contact with cytochrome c. FEBS Lett. (1982) 150, 49-53
  33. TOMASI M., D’AGNOLO G. & MONTECUCCO C. Micellar gangliosides mediate the lipid insertion of cholera toxin protomer A. Biochim. Biophys. Acta (1982) 692, 339-344
  34. MONTECUCCO C., SMITH G.A., DABBENI-SALA F., JOHANNSSON A., GALANTE Y.M. & BISSON R. Bilayer thickness and enzymatic activity in the mitochondrial cytochrome c oxidase and ATPase complex. FEBS Lett. (1982) 144, 145-148
  35. GUTWENIGER H., BISSON R. & MONTECUCCO C. Membrane topology of beef-heart ubiquinone-cytochrome c reductase (complex III). J. Biol. Chem. (1981) 256, 11132-11136
  36. TOMASI M. & MONTECUCCO C. Lipid insertion of cholera toxin after binding to GM1-containing liposomes. J. Biol. Chem. (1981) 256, 11177-11181
  37. MONTECUCCO C., BISSON R., GACHE C. & JOHANNSSON A. Labelling of the hydrophobic domain of the (Na+, K+)-ATPase. FEBS Lett. (1981) 128, 17-21
  38. MONTECUCCO C., BALLARDIN S., ZACCOLIN G.P. & POZZAN T. Effect of local anesthetics on lymphocyte capping and energy metabolism. Biochem. Pharmacol. (1981) 30, 2989-2992
  39. ARSLAN P., MONTECUCCO C., CELI D. & POZZAN T. Effect of monovalent cation ionophores on rat spleen lymphocyte metabolism. Biochim. Biophys. Acta (1981) 643, 177-181
  40. GUTWENIGER H., BISSON R. & MONTECUCCO C. Hydrophobic photolabelling of the yeast cytochrome c oxidase subunits in contact with lipids. Biochim. Biophys. Acta (1981) 635, 187-193
  41. BISSON R. & MONTECUCCO C. Photolabelling of membrane proteins with photoactive phospholipids. Biochem. J. (1981) 193, 757-763
  42. POZZAN T., CORPS A.N., MONTECUCCO C., HESKETH T.R. & METCALFE J.C. Cap formation by various ligands on lymphocytes show the same dependence on high cellular ATP levels. Biochim. Biophys. Acta (1980) 602, 558-566
  43. MONTECUCCO C., RINK T.J., POZZAN T. & METCALFE J.C. Triggering of lymphocyte capping appears not to require changes in potential or ion fluxes across the plasma membrane. Biochim. Biophys. Acta (1980) 595, 65-70
  44. RINK T.J., MONTECUCCO C., HESKETH T.R. & TSIEN R.Y. Lymphocyte membrane potential assessed with fluorescent probes. Biochim. Biophys. Acta (1980) 595, 15-30
  45. MONTECUCCO C., BISSON R., DABBENI-SALA F., PITOTTI A. & GUTWENIGER H. Interaction of the mitochondrial ATPase complex with phospholipids. J. Biol. Chem. (1980) 255, 10040-10043
  46. MONTECUCCO C., GUTWENIGER H., JOHANNSSON A. & BISSON R. Hydrophobic photolabelling of sodium-plus-potassium ion stimulated adenosine triphosphatase. Biochem. Soc. Trans. (1979) 7, 952-953
  47. MONTECUCCO C., BISSON R., PITOTTI A., DABBENI-SALA F. & GUTWENIGER H. Labeling of the subunits of the mitochondrial adenosine triphosphatase complex in contact with the lipid bilayer. Biochem. Soc. Trans. (1979) 7, 954-955
  48. BISSON R., MONTECUCCO C., GUTWENIGER H. & AZZI A. Cytochrome c oxidase subunits in contact with phospholipids: hydrophobic photolabeling with azidophospholipids. J. Biol. Chem. (1979) 254, 9962-9965
  49. BISSON R., MONTECUCCO C. & CAPALDI R.A. Interaction of membranous cytochrome b5 with arylazidophospholipids. FEBS Lett. (1979) 106, 317-320
  50. MONTECUCCO C., POZZAN T. & RINK T.J. Dicarbocyanine fluorescent probes of membrane potential block lymphocyte capping, deplete cellular ATP and inhibit respiration of isolated mitochondria. Biochim. Biophys. Acta (1979) 552, 552-557
  51. BISSON R., MONTECUCCO C., GUTWENIGER H. & AZZI A. Hydrophobic labelling of bovine heart cytochrome c oxidase with azidophosphatidylcholine. Biochem. Soc. Trans. (1979) 7,156-159
  52. MacINTYRE D.E., MONTECUCCO C. & RINK T.J. Platelet membrane potential assessed with a fluorescent probe, dipropylthiacarbocyanine: has this potential a role in triggering aggregation? J. Physiol. (1978) 289, 34-35p
  53. SMITH G.A., MONTECUCCO C. & BENNETT J.P. Isotopic labeling of phosphatidylcholine in the choline moiety. Lipids (1978) 13, 92-94
  54. BISSON R., AZZI A., GUTWENIGER H., COLONNA R., MONTECUCCO C. & ZANOTTI A. Interaction of cytochrome c with cytochrome c oxidase: photoaffinity labelling of beef heart cytochrome oxidase with arylazido-cytochrome c. J. Biol. Chem. (1978) 253, 1874-1880
  55. BISSON R., GUTWENIGER H., MONTECUCCO C., COLONNA R., ZANOTTI A. & AZZI A. Covalent binding of arylazido derivatives of cytochrome c to cytochrome oxidase. FEBS Lett. (1977) 81, 147-150
  56. AZZI A. & MONTECUCCO C. Probes for energy transduction in membranes. J. Bioenerg. Biomembr. (1978) 8, 257-269
  57. AZZI A., MONTECUCCO C. & RICHTER C. The use of acetylated ferricytocchrome c for the detection of superoxide radicals in biological membranes. Biochem. Biophys. Res. Commun. (1975) 65, 597-603
  58. MONTECUCCO C. & AZZI A. Molecular interactions of adenosine triphosphatase with the mitochondrial membrane as revealed by a spin label study. J. Biol. Chem. (1975) 250, 5020-5025



  1. Rappuoli, R. and Montecucco, C. (Editors). Protein Toxins and their Use in Cell Biology. Oxford University Press, Oxford 1997
  2. Montecucco C. (Editor). Clostridial Neurotoxins. Springer Verlag Berlin and Heidelberg, vol 195, 1995
  3. Mock, M. and Montecucco, C. (Editors) Anthrax. Molecular Aspects of Medicine vol. 30 (2009)

Reviews, Hypothesis, Position Papers, Interviews


  1. Megighian, A, Pirazzini, M, Fabris, F, Rossetto, O and Montecucco, C. Tetanus and Tetanus Neurotoxin: from peripheral uptake to central nervous tissue targets. J. Neurochem


  1. Eleopra R, Rinaldo S, Montecucco C, Rossetto O, Devigili G. Clinical duration of action of different botulinum toxin types in humans. Toxicon. 2020 pii: S0041-0101(20)30065-9. doi: 10.1016/j.toxicon.2020.02.020. [Epub ahead ofprint] PubMed PMID: 32184153.


  1. Rossetto O, Pirazzini M, Fabris F, Montecucco C. Botulinum Neurotoxins: Mechanism of Action. Handb Exp Pharmacol. 2020 , doi: 10.1007/164_2020_355.[Epub ahead of print] PubMed PMID: 32277300


  1. Rossetto O, Pirazzini M, Lista R, Montecucco C. The role of the single interchains disulfide bond in tetanus and botulinum neurotoxins and the development of antitetanus and antibotulism drugs. Cell. Microbiol. 2019; e13037. https://doi.org/10.1111/cmi.13037


  1. Montecucco C. An Interview with Cesare Montecucco. Toxins (2018) doi:10.3390/toxins10070000


  1. Doxey AC, Mansfield MJ, Montecucco C. Discovery of novel bacterial toxins by genomics and computational biology. Toxicon (2018) 147:2-12. doi: 10.1016/j.toxicon.2018.02.002.
  2. Pirazzini M, Azarnia Tehran D, Zanetti G, Rossetto O, Montecucco C. Hsp90 and Thioredoxin-Thioredoxin Reductase enable the catalytic activity of Clostridial neurotoxins inside nerve terminals. Toxicon (2018) 147:32-37. doi: 10.1016/j.toxicon.2017.10.028.
  3. Pirazzini M, Rossetto O, Eleopra R. & Montecucco C. Botulinum Neurotoxins: biology, pharmacology and toxicology. Pharmacol. Rev. (2017) 69:200-235.
  4. Rigoni M & Montecucco C. Animal Models to Study Motor Axon Terminal Paralysis and Recovery. J. Neurochem. (2017) Suppl 2:122-129 doi: 10.1111/jnc.13956.
  5. Montecucco C, Zanotti G. Botulinum neurotoxin A1 likes it double sweet. Nat Struct Mol Biol. (2016) 23:619-21, doi: 10.1038/nsmb.3253.
  6. Carafoli E, Montecucco C. Research funding: Reform oversight of Italy’s science funds. Nature (2016) 533:179. doi: 10.1038/533179c.
  7. Pirazzini M, Tehran DA, Leka O, Zanetti G, Rossetto O, Montecucco C. On the translocation of botulinum and tetanus neurotoxins across the membrane of acidic intracellular compartments. Biochim Biophys Acta (2016) 1858:467-474.
  8. Rossetto O, Pirazzini M, Montecucco C. Current gaps in basic science knowledge of botulinum neurotoxin biological actions. Toxicon (2015) 107:59-63. doi: 10.1016/j.toxicon.2015.07.002.
  9. Pirazzini M, Tehran DA, Zanetti G, Lista F, Binz T, Shone CC, Rossetto O, Montecucco C. The thioredoxin reductase – Thioredoxin redox system cleaves the interchain disulphide bond of botulinum neurotoxins on the cytosolic surface of synaptic vesicles. Toxicon. (2015) 107:32-6. doi:10.1016/j.toxicon.2015.06.019.
  10. Albanese A, Abbruzzese G, Dressler D, Duzynski W, Khatkova S, Marti MJ, Mir P, Montecucco C, Moro E, Pinter M, Relja M, Roze E, Skogseid IM, Timerbaeva S, Tzoulis C. Practical guidance for CD management involving treatment of botulinum toxin: a consensus statement. J. Neurol. (2015) 262:2201-13. doi: 10.1007/s00415-015-7703-x
  11. Montecucco C. and Rasotto M.B. On Botulinum Neurotoxin Variability. (2015) MBio pii: e02131-14. doi: 10.1128/mBio.02131-14
  12. Rossetto O., Pirazzini M., Montecucco C. Botulinum neurotoxins: recent genetic, structural and mechanistic insights Nat. Rev. Microbiol. (2014) 12:535-49. doi: 10.1038/nrmicro3295
  13. Pantano S. & Montecucco C. The botulinum neurotoxins and the neuroexocytosis apparatus. Cell. Mol. Life Sci. (2014) 71:793-811. doi: 10.1007/s00018-013-1380-7.
  14. Rossetto O, Megighian A, Scorzeto M, Montecucco C. Botulinum neurotoxins.

Toxicon. (2013) 67:31-36. doi: 10.1016/j.toxicon.2013.01.017.

  1. Rossetto O, Scorzeto M, Megighian A, Montecucco C. Tetanus neurotoxin.

Toxicon. (2013) 66:59-63. doi: 10.1016/j.toxicon.2012.12.027.

  1. Montecucco, C & Tonello, F. Bontoxilysins, in The Handbook of Proteolytic Enzymes. Neil D. Rawlings and Guy S. Salvesen, editors,. Oxford: Academic Press, (2013), pp. 660-665.
  2. Montecucco, C & Rossetto, O. Tentoxilysin, in The Handbook of Proteolytic Enzymes. Neil D. Rawlings and Guy S. Salvesen, editors,. Oxford: Academic Press, (2013), pp. 655-6600.
  3. Davletov B, Montecucco C. Lipid function at synapses. Curr Opin Neurobiol. (2010) 20:543-549.
  4. Tonello F, Montecucco C. The anthrax lethal factor and its MAPK kinase-specific metalloprotease activity. Mol Aspects Med. (2009) 30:431-8. doi: 10.1016/j.mam.2009.07.006.
  5. Rossetto O, Morbiato L, Caccin P, Rigoni M, Carli L, Paoli M, Cintra-Francischinelli M and Montecucco C. Tetanus, Botulinum and Snake Presynaptic Neurotoxins. Rendiconti Lincei (2008) 19, 173 – 188.
  6. Johnson E.A. & Montecucco C.  Botulism. Handb Clin Neurol. (2008) 91:333-368.
  7. Montecucco C., Gutierrez J.M. & Lomonte B. Cellular pathology induced by snake venom phospholipase A2 myotoxins and neurotoxins: common aspects of their mechanisms of action. Cell. Mol. Life Sci. (2008) 65:2897-2912
  8. Montecucco C. & Rossetto O.   On the quaternary structure of taipoxin and textilotoxin: the advantage of being multiple. Toxicon (2008)  51:1560-1562
  9. Hudson MJ, Beyer W, Böhm R, Fasanella A, Garofolo G, Golinski R, Goossens PL,

Hahn U, Hallis B, King A, Mock M, Montecucco C, Ozin A, Tonello F, Kaufmann SH.

Bacillus anthracis: balancing innocent research with dual-use potential. Int J

Med Microbiol. (2008) 298:345-364.

  1. Rossetto O. & Montecucco C. Presynaptic Neurotoxins with Enzymatic Activities.

Handb Exp Pharmacol. (2008) 184:129-170.

  1. ROSSETTO O. & MONTECUCCO C. Peculiar binding of botulinum neurotoxins. ACS Chem. Biol. (2007) 2, 96-98
  2. VERDERIO C., ROSSETTO O., GRUMELLI C., FRASSONI C., MONTECUCCO C. & MATTEOLI M. Synaptic vesicles recycling is used by botulinum toxins to enter inside neurons. EMBO Rep. (2006) 7, 995-999.
  3. ELEOPRA R., TUGNOLI V., QUATRALE R., ROSSETTO O., MONTECUCCO C. & DRESSLER D. Clinical use of non-A botulinum toxins: botulinum toxin type C and botulinum toxin type F. Neurotox Res. (2006) 9, 127-131.
  4. Baldari CT, Tonello F, Paccani SR, Montecucco C. Anthrax toxins: A paradigm of

bacterial immune suppression. Trends Immunol. (2006) 27:434-40. Epub 2006

Jul 24. Review.

  1. ROSSETTO O., MORBIATO L., CACCIN P., RIGONI M. & MONTECUCCO C. Presynaptic Enzymatic Neurotoxins. J. Neurochem. (2006) 97,1534-1545.
  2. MONTECUCCO, C., SCHIAVO, G. & PANTANO S. SNARE complexes and neuroexocytosis: how many, how close ? Trends Biochem. Sci. (2005) 30, 367-372
  3. MONTECUCCO, C. & MOLGO J. Botulinum neurotoxins: revival of an old killer. Curr. Op. Pharmacol. (2005) 5, 274-279.
  4. ROSSETTO O., RIGONI M. & MONTECUCCO C. Different mechanism of blockade of neuroexocytosis by presynaptic neurotoxins. Toxicol. Lett. (2004) 149, 91-101.
  5. MONTECUCCO, C., ROSSETTO, O. & SCHIAVO, G. Presynaptic receptor arrays for clostridial neurotoxins. Trends Microbiol. (2004) 12, 442-446.
  6. MONTECUCCO, C., TONELLO, F. & ZANOTTI G. Stop the killer: how to inhibit the anthrax lethal factor metalloprotease. Trends Biochem. Sci. (2004), 29, 282-285
  7. MONTECUCCO, C. & DE BERNARD, M. Immunosoppressive and Proinflammatory Activities of the VacA Toxin of Helicobacter pylori. J. Exp. Med. (2003) 198, 1767-1771.
  8. MONTECUCCO, C. & DE BERNARD, M. Molecular and cellular mechanisms of action of the vacuolating cytotoxin (VacA) and neutrophil-activating protein (HP-NAP) virulence factors of Helicobacter pylori. Microbes and Infection (2003) 5, 715 – 721.
  9. ROSSETTO, O., CACCIN, P., SEVESO, M. & MONTECUCCO, C. Botulinum neurotoxins are metalloproteases specific for SNARE proteins. Curr. Probl. Dermatol. (2002) 30, 117-125.
  10. ASCENZI P., VISCA P., IPPOLITO G., SPALLAROSSA A., BOLOGNESI M. & MONTECUCCO C. Anthrax toxin: a tripartite lethal combination. FEBS Lett. (2002) 531, 384-388.
  11. DUNDON, W.G., NISHIOKA, H., POLENGHI, A., PAPINUTTO, E., ZANOTTI, G., MONTEMURRO, P., DEL GIUDICE, G., RAPPUOLI, R. & MONTECUCCO, C. The neutrophil-activating protein of Helicobacter pylori. Int. J. Med. Microbiol. (2002) 291, 545-550.
  12. ROSSETTO, O., SEVESO, M., CACCIN, P, SCHIAVO, G. and MONTECUCCO, C. Tetanus and botulinum neurotoxins: turning bad guys into good by research. Toxicon. (2001) 39, 27-41
  13. MONTECUCCO, C. Detoxification of a bacterial toxin by the toxin itself. Trends Pharmacol. Sci. (2001) 22, 493-494.
  14. MONTECUCCO, C. & RAPPUOLI, R. Living dangerously: how Helicobacter pylory survives in the human stomach. Nature Rev. Cell Biol. (2001) 2, 457-466.
  15. DUNDON, W., NISHIOKA, H., MONTECUCCO, C., MONTEMURRO, P., RAPPUOLI, R. & DEL GIUDICE, G. Problems in assaying neutrophil activators. Trends Microbiol. (2001) 9, 314-315
  16. DUNDON, W., DE BERNARD, M. & MONTECUCCO, C. Virulence factors of Helicobacter pylori. Int. J. Med. Microbiol. (2001) 290, 647-658.


  1. DEL GIUDICE G., COVACCI A., TELFORD JL., MONTECUCCO C. & RAPPUOLI R. The design of vaccines against Helicobacter pylori and their development. Annu Rev. Immunol. (2001) 19, 523-563.
  2. MONTECUCCO, C., PAPINI, E., DE BERNARD, M. & ZORATTI, M. The anion-specific channel of Helicobacter pylori cytotoxin VacA. Curr. Top. Microbiol. Immunol. (2001) 257, 113-129.
  3. MONTECUCCO, C. From dreaded pathogen to therapeutic agent. Science Spectra (2000) 19, 62-69.
  4. MONTECUCCO, C. & ROSSETTO, O. How do presynaptic PLA2 neurotoxins block nerve terminals ? Trends Biochem. Sci.. (2000) 25, 266-270
  5. SCHIAVO, G., MATTEOLI, M. & MONTECUCCO, C. Neurotoxins Affecting Neuroexocytosis. Physiol. Rev. (2000) 80, 717-766.
  6. MONTECUCCO, C., PAPINI, E., DE BERNARD, M. & ZORATTI, M. Molecular and cellular activities of Helicobacter pylori pathogenic factors. FEBS Lett. (1999) 452, 16-21.
  7. REYRAT, J.M., PELICIC, V., PAPINI, E., MONTECUCCO, C., PAPPUOLI, R. & TELFORD, J.L. Towards the deciphering of the Helicobacter pylori cytotoxin. Mol. Microbiol. (1999), 34, 197-204
  8. PELLIZZARI, R., ROSSETTO, O., SCHIAVO, G. & MONTECUCCO, C. Tetanus and botulinum neurotoxins: mechanism of action and therapeutic uses. Proc. Phyl. Trans. R. Soc. Lond. [Biol] (1999) 354, 259-268
  9. MONTECUCCO, C. Protein Toxins and Membrane Transport. Curr. Op. Cell Biol. (1998) 10, 530-536.
  10. WASHBOURNE, P., PELLIZZARI, R., ROSSETTO, O., BORTOLETTO, N., TUGNOLI, V., DE GRANDIS, D., ELEOPRA, R. and MONTECUCCO, C. On the action of botulinum neurotoxins A and E at cholinergic terminals. J. Physiol. (Paris) (1998) 92, 135-139.
  11. TUGNOLI V., ELEOPRA R., MONTECUCCO, C. & DE GRANDIS D. The therapeutic use of botulinum toxin. Exp. Opin. Invest. Drugs (1997) 6, 1383-1394
  12. MATTEOLI, M. & MONTECUCCO, C. Le neurotossine presinaptiche. Le Scienze n. 350, 59, pp. 92-104, (1997)
  13. MONTECUCCO, C, PAPINI, E & SCHIAVO, G. Bacterial Protein Toxins and Cell Vesicle Trafficking. Experientia (1996) 52, 1026-1032.
  14. MONTECUCCO C., SCHIAVO G., TUGNOLI, V. & DE GRANDIS, D. Botulinum Neurotoxins: mechanism of action and therapeutic applications. Molecular Medicine Today (1996), 2, 418-424.
  15. SCHIAVO G., ROSSETTO O., MORANTE S., TONELLO F., PELLIZZARI R. & MONTECUCCO C. Tetanus and botulinum neurotoxins: a new group of metallo-proteinases. Bio. It. (1995) 3, 27-43
  16. MONTECUCCO C. & PAPINI E. Cell penetration of bacterial protein toxins. Trends Microbiol. (1995) 3, 165-167
  17. MONTECUCCO C. & SCHIAVO G. Structure and function of Tetanus and Botulinum Neurotoxins. Quart. Rev. Biophys. (1995), 28, 423-478
  18. SCHIAVO G., ROSSETTO O., TONELLO F. & MONTECUCCO, C. The metalloproteinase activity of tetanus and botulinum neurotoxins. Curr. Top. Microbiol. Immunol. (1995) 195, 257-274.
  19. ROSSETTO O., DELOYE F., POULAIN B., PELLIZZARI R., SCHIAVO G. & MONTECUCCO, C. The metallo-proteinase activity of tetanus and botulism neurotoxins. J. Physiol. (Paris) (1995) 89, 43-50
  20. MONTECUCCO C. & SCHIAVO, G. Mechanism of action of tetanus and botulism neurotoxins. Mol. Microbiol. (1994) 13, 1-8
  21. MONTECUCCO C., PAPINI E. & SCHIAVO G. Bacterial protein toxins penetrate cells via a four-step mechanism. F.E.B.S. Letters (1994) 346, 92-98
  22. TELFORD J.L., COVACCI A., GHIARA P., MONTECUCCO C. & RAPPUOLI R. Unravelling the pathogenic role of Helicobacter pylori in peptic ulcer: potential new therapies and vaccines. Trends Biotechnol. (1994) 12, 420-426.
  23. CHERINGTON M., SMITH R.H. & MONTECUCCO, C. Microbial Toxins. Handbook Clinical Neurology (1994) 21, 1-40.
  24. MENESTRINA G., SCHIAVO G. & MONTECUCCO, C. Molecular mechanisms of action of bacterial protein toxins. Molec. Aspects Med. (1994) 15, 79-193. 139.
  25. SCHIAVO G., ROSSETTO O. & MONTECUCCO C. Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter release. Seminars Cell. Biol. (1994) 5, 221-229.
  26. SCHIAVO, G., ROSSETTO, O., BENFENATI, F., POULAIN, B. and C. MONTECUCCO Tetanus and botulinum neurotoxins are zinc proteases specific for components of the neuroexocytosis apparatus. Ann. N.Y. Acad. Sci. (1994) 710, 65-76.
  27. MONTECUCCO C. & SCHIAVO G. Tetanus and Botulinum Neurotoxins: a new group of zinc-endopeptidases. Trends Biochem. Sci. (1993) 18, 324-327.
  28. SCHIAVO G., POULAIN B., BENFENATI F., DAS GUPTA B.R. & MONTECUCCO, C. Novel target and catalytic activity of bacterial protein toxins. Trends Microbiol. (1993) 1, 170-174.
  29. SCHIAVO, G., ROSSETTO, O. & MONTECUCCO, C. Le basi molecolari del tetano e del botulismo. Le Scienze (1993) 304, 40-48.
  30. SCHIAVO G., BOQUET P., DASGUPTA B.R. & MONTECUCCO C. Membrane interactions of tetanus and botulinum neurotoxins: a photolabelling study with photoactivatable phospholipids. J. Physiol. (Paris) (1990) 84, 180-187
  31. MONTECUCCO C., PAPINI E. & BISSON R. Protein-lipid interactions. Ann. Ist. Super. Sanit (1988) 24, 3-8
  32. MONTECUCCO C., PAPINI E., SCHIAVO G., RAPPUOLI R. & TOMASI M. Studies on the interaction of diphtheria toxin into biological membranes. Zentr. Bakteriol. Mikrobiol. Hyg. (1988) 17, 287-294
  33. BISSON R. & MONTECUCCO C. Diphtheria toxin membrane translocation: an open question. Trends Biochem. Sci. (1987) 12, 187-188
  34. MONTECUCCO, C. How do tetanus and botulinum toxins bind to neuronal membranes? Trends Biochem. Sci. (1986) 11, 314-317.
  35. MONTECUCCO C., RAPPUOLI R. & TOMASI M. Tossine batteriche a bersaglio intracellulare. Aggiornamento del Medico (1986) 10, 206-213.
  36. TOMASI M., GALLINA A., D’AGNOLO G. & MONTECUCCO, C. Interaction of cholera toxin with lipid model membranes. Zentr. Bakteriol. Mikrobiol. Hyg. (1986) 15, 19-25.
  37. AZZI A. & MONTECUCCO C. Spin labels and biological membranes. Horizons Biochem. Biophys. (1978) 4, 266-294

Book Chapters

  1. ROSSETTO O. & MONTECUCCO C. (2004). Clostridial neurotoxins. In: Microbial Toxins: Molecular and Cellular Biology. T. Proft editor. chapter 7, pp.149-178 Horizon Bioscience, Norfolk, UK.
  2. DE BERNARD, M. & MONTECUCCO, C. (2003). Microbial Subversion of Host Cells. pp.177-198. Cambridge University press, Cambridge, UK.


  1. MONTECUCCO, C. Neurotoxins. Wiley Encyclopedia of Molecular Medicine, vol. 5, 2260-2261 (2002).
  2. MONTECUCCO, C. Diphtheria toxin. Wiley Encyclopedia of Molecular Medicine, vol. 5, 1057-1058 (2002).
  3. Rossetto O., Tonello F. and Montecucco C. (2002). Proteases. In: Bacterial Protein Toxins (eds. Barbieri J., Rappuoli R., Iglewski B. and Burns D.). American Society for Microbiology Press. pp.271-282.
  4. Rossetto O., Caccin P., Rigoni M., Seveso M., Tonello F., Stevens R.C. and Montecucco C. (2002). The metalloprotease activity of tetanus and botulinum neurotoxins. In: Scientific and therapeutic aspect of botulinum toxin” (eds Jankovic J., Hallett M. and Brin M.F.). pp. 3-10. Lippincott Williams and Wilkins, Philadelphia.
  5. ROSSETTO O. & Montecucco C. (2002). The molecular basis of the activity of botulinum neurotoxins. In: Handbook of botulinum toxin treatment 2nd edition, (eds. Moore AP and Naumann M). Blackwell Science Ltd. Pp. 9-27.
  6. Rossetto O., Seveso M., Caccin P. and Cesare Montecucco (2002). Botulinum neurotoxins are metalloproteases specific for SNARE proteins involved in neuroexocytosis. In: Hyperhidrosis and Botulinum in Dermatology (eds: Kreyden O., Böni and Burg G.) Curr. Probl. Dermatol., Basel Karger. In press.
  7. Rossetto O. and Montecucco C. (2002). Bacterial toxins with metalloprotease activity. Perspectives in Molecular Toxinology (ed. A. Menez). Chapter 1, pp. 3-22.John Wiley & Sons, Ltd., Chichester, UK.
  8. de Bernard M., Rossetto O. and Montecucco C. (2001). Bacterial toxin: intracellular trafficking and target identification. In: “Molecular Cellular Microbiology” (eds. P. Sansonetti and A. Zichlinscky). Academic Press, London. Chapter 16, 297-317.
  9. O. Rossetto, G. Schiavo and C. Montecucco. Clostridium tetani and tetanus neurotoxin. Mol. Med. Microbiol. (ed. M. Sussman) (2001), Academic Press, Chapter 90, 1905-1919.
  10. MONTECUCCO, C. & TONELLO, F. (2002) Botulinum Neurotoxins. Handbook of Proteolytic Enzymes, Academic press, in press
  11. MONTECUCCO, C. & ROSSETTO, O. Tetanus Neurotoxin. Handbook of Proteolytic Enzymes, Academic press, in press
  12. MONTECUCCO, C., PAPINI, E., DE BERNARD, M., DUNDON, W.G., ZORATTI, M., REYRAT, J-M-, TELFORD, J.L., DEL GIUDICE, G. and RAPPUOLI, R. Helicobacter pylori vacuolating cytotoxin and neutrophil activating protein. In Helicobacter pylori: Molecular and cellular Biology (2001) (S. Suerbaum & M. Achtman, Editors), Horizon Scientific Press, pp. 245-262, Norfolk (UK).
  13. SCHIAVO, G. & MONTECUCCO, C. Tossine batteriche. In “Tossicologia Molecolare e Cellulare. Cantelli Forti, G., Galli, C.L., Hrelia, P. & Marinovich, M. Editori, pp. 149-159 (2000). UTET, Torino.
  14. MONTECUCCO, C., PAPINI, E., DE BERNARD, M., TELFORD, J.E. & RAPPUOLI, R. Helicobacter pylori vacuolating cytotoxin and associated pathogenic factors. . In Bacterial protein Toxins: a sourcebook. (J.E. Alouf & J.H. Freer Eds. ) Academic press, (1999) pp. 264-283, New York
  15. HERREROS, J., LALLI, G., MONTECUCCO, C. & SCHIAVO, G. Pathophysiological properties and mechanism of action of clostridial neurotoxins. In Bacterial protein Toxins: a sourcebook. (J.E. Alouf & J.H. Freer Eds. ) Academic Press, (1999) pp. 202-228, New York
  16. MONTECUCCO, C., PELLIZZARI, R., ROSSETTO, O., SCHIAVO, G., TONELLO, F. & WASHBOURNE, P. Clostridial Neurotoxins as Enzymes: Structure and Function. In “Cellular and Molecular Mechanisms of Toxin Action on Secretory Systems” (Linial, M & Grasso, A. Eds.) (1998), pp.315-321. Harwood Academic Publishers, Amsterdam
  17. SCHIAVO, G. & MONTECUCCO, C. The structure and mode of action of botulinum and tetanus toxins. in The Clostridia Molecular Biology and Pathogenesis (Rood, J., Songer, G., McClane, B. & Titball, R. Eds) (1997), pp. 295-322. Academic Press, San Diego
  18. SCHIAVO, G., ROSSETTO, O. & MONTECUCCO, C. Tetanus and botulinum neurotoxins. in Zinc Metalloproteases in Health and Disease (Hooper, N.M. Ed.), (1996), pp. 205-220, Taylor & Francis, London
  19. MONTECUCCO, C., SCHIAVO, G. & ROSSETTO, O. The mechanism of action of tetanus and botulinum neurotoxins. in “Archiv. Toxicol. Suppl. 18”, (Seiler, J.P., Kroftova, O. & Eybl., V. Editors), pp. 342-354, Springer Verlag, Berlin.1996
  20. TONELLO F., MORANTE S., SCHIAVO G., ROSSETTO O. & MONTECUCCO C. Tetanus and Botulism neurotoxins: a novel group of zinc-endopeptidases. in “10th ICOP Intracellular Protein Catabolism Conference” (Suzuki, K. and Bond, J. Eds), pp. 251-260 (1995) Plenum Publishing Co., New York
  21. TONELLO F., SCHIAVO G., ROSSETTO O. & MONTECUCCO C. Tetanus and Botulism neurotoxins. in “Astacin Family of Metalloendopeptidases”, (Zwilling, R. and Stocker, W. Eds), pp. 176-182 (1995), University of Heidelberg Press, Heidelberg.
  22. MONTECUCCO C., SCHIAVO G., PAPINI E., TOMASI M. & BISSON R. Membrane protein labelling with photoactive phospholipid analogues. in “Photochemical Probes in Biochemistry” (Nielsen, P.E. Ed.), pp. 43-58, NATO ASI Series, Kluwer Academic Publ., Dordrecht, 1989.
  23. MONTECUCCO C.Theoretical considerations on the cellular mechanism of action of clostridial neurotoxins. in “Eight International Conference on Tetanus”, (Nisticò, G., Bizzini, B., Bytchenko, B. and Triau, R. Eds.), pp. 71-91, Pythagora Press, Rome, 1989.
  24. GIRAUDAT J., DENNIS M., HEIDMANN T., CHANGEUX J.P., BISSON R., MONTECUCCO C., KOTZYBA-HIBERT F., GOELDNER M., HIRTH C., & CHANG J.Y. Tertiary structure of the nicotinic acetylcholine receptor probed by photolabeling and protein chemistry. in “Nicotinic acethylcholine receptor”, Maelike, A. Ed. NATO Asi Series, H 3 pp. 104-114, Springer-Verlag, Berlin (1986).
  25. MONTECUCCO, C. Hydrophobic photolabelling with 125I-TID of red blood cell membranes. in “Isolation and Characterization of membrane proteins: biochemical and biophysical aspects”, Azzi, A., Masotti, L. and Vecli, A. Eds., pp. 119-123, Springer-Verlag, Berlin
  26. BISSON R. & MONTECUCCO C. Topology of membrane proteins: determination of regions exposed to the lipid bilayer. in “Techniques for the analysis of membrane proteins”, pp. 153-184, Cherry, R. and Ragan, C.I. Eds., Chapman and Hall, London (1986).
  27. BISSON R. & MONTECUCCO C. Use of photoreactive phospholipids for the study of lipid-protein interactions. In “Progress in Protein-lipid Interactions”, pp. 259-287, Watts A. and de Pont J.J.H.H.M. Eds., Elsevier Sceince Publishers B.V., Amsterdam (1985)
  28. MONTECUCCO C., FRIEDL P. & HOPPE J. A hydrophobic photolabelling study of the ATP synthase from E. coli. in “Structure and Function of Membrane Proteins”, pp. 91-94, Quagliariello E. and Palmieri F. Eds., Elsevier Science Publ. Co., Amsterdam (1983)
  29. MONTECUCCO C. & BISSON R. Lipid-protein interaction: a hydrophobic photolabelling study. in “Transport in Biomembranes Model Systems and Reconstitution” pp. 145-151, Antolini R. et al., eds., Raven Press, New York (1982)
  30. ARSLAN P., MIOLO A., MONTECUCCO C. & POZZAN T. Effect of ionophores on lymphocyte cellular metabolism. in “Biochemistry and Function of Phagocytes”, Rossi F. and Patriarca P.L. Eds., Adv. Experimental Medicine and Biology (1982) 141, 481-489
  31. MONTECUCCO C., BISSON R., ZACCOLIN G.P., DABBENI-SALA F. & GALANTE Y.M. A high resolution hydrophobic photolabelling study of the mitochondrial ATPase F sector. in “Vectorial Reactions in Electron and Ion Transport in Mitochondria and Bacteria” pp. 227-230, Palmieri F. et al., Eds., Elsevier North-Holland Biomedical Press, Amsterdam (1981)
  32. AZZI A., BISSON R., CASEY R.P., CHAPPELL J.B., GUTWENIGER H., MONTECUCCO C. & SIGRIST-NELSON K. Structural and functional properties of cytochrome c oxidase. in “Frontiers of Bioorganic and Molecular Biology” pp. 53-62, Ananchivo S.N. Ed., Pergamon Press, Oxford (1980)
  33. ARSLAN P., MONTECUCCO C., MIOLO A. & POZZAN T. Effect of monovalent cation ionophores on cellular metabolism of spleen lymphocytes. in “Function and Molecular Aspects of Biomembrane Transport” pp. 75-78, Quagliariello E. et al., Eds. Elsevier North-Holland Biomedical Press, (1979)
  34. AZZI A., BISSON R., CASEY R.P., GUTWENIGER H., MONTECUCCO C. & THELEN M. Hydrophobic interactions, proton movements and dicyclohexylcarbodiimide labeling in cytochrome c oxidase. in “Membrane Bioenergetics” pp. 13-20, Lee C.P. et al., Eds., Addison-Wesley Publ. Co., (1979)
  35. BISSON R., MONTECUCCO C., GUTWENIGER H. & AZZI A. Photoaffinity labels for the study of cytochrome c and phospholipid interactions with cytochrome c oxidase. in “Frontiers of Biological Energetics”, vol. 2, pp. 809-816, Scarpa A. et al. Eds., Academic Press, New York (1978)
  36. MONTECUCCO C., SMITH G.A., WARREN G.A. & METCALFE J.C. A spin label assay of the annular lipids around a calcium transport protein. in “Structure and Function of Energy-Transducing Membranes”, pp. 187-191, Van Dam K. and Van Gelder, B.F. Eds., Elsevier North-Holland Biomedical Press, Amsterdam (1977)
  37. BISSON R., GUTWENIGER H., MONTECUCCO C., COLONNA R., ZANOTTI S. & AZZI A. Photoaffinity binding of cytochrome c to cytochrome c oxidase. in “Bioenergetics of Membranes” pp. 39-46, Packer L. et al., Eds., North-Holland Biomedical Press, Amsterdam (1977)
  38. AZZI A., SORGATO C.M. & MONTECUCCO C. Effect of calcium ions on the interaction between cytochrome c and the mitochondrial membrane. in “Calcium Transport in Contraction and Secretion”, pp. 35-44, Carafoli E. et al., Eds., North-Holland Publ. Co., Amsterdam (1975)
  39. AZZI A. & MONTECUCCO C. Interaction of the mitochondrial adenosintriphosphatase with the membrane: molecular information obtained with a spin-label inhibitor. in “Electron Transfer Chains and Oxidative Phosphorylation” pp. 173-178, Quagliariello E. et al., Eds., North-Holland Publ. Co., Amsterdam (1975)
  40. AZZI A., MONTECUCCO C. & SANTATO M. The interaction of site specific spin labels (nitroxide-cytochrome c and nitroxide-carbodiimide) with the mitochondrial membrane. in “Membrane Proteins in Transport and Phosphorylation” pp. 205-210, Azzone G.F. et al., Eds., North-Holland Publ. Co., Amsterdam (1974)